Label-Free Optical Biosensors for Monitoring Cellular Processes and Cytotoxic Agents at Interfaces Using Guided Modes and Advanced Phase-Contrast Imaging Techniques

Part of the Advanced Sciences and Technologies for Security Applications book series (ASTSA)


Novel optical biosensors in direct interaction with living cells open new avenues in the detection of given cell types, including pathogenic microorganisms, and in the measurement of cytotoxicity of given xenobiotics, such as biothreat agents, on suitably selected cell types or lines. Evanescent optical field based biosensors emerging for such applications are reviewed, covering surface plasmon and waveguide based formats, imaging setup, employing optical waveguides, and digital holographic microscopy. Commercial technologies, along with main pathogenic microorganisms (Bacillus anthracis, Escherichia coli, Listeria monocytogenes, Campylobacter jejuni, etc.) as analytes and cell lines (native tissue and tumor cells) as cytotoxicity effect subjects are highlighted.


Evanescent field Cell biosensor Optical waveguide Pathogenic microorganisms Cytotoxicity Digital holographic microscopy 



Research was funded by projects “Development of optical bio-sensors for detection of bio-toxins” (NUKR.SFPP 984637) in the NATO SPS Programme, “Mechanism-related teratogenic, hormone modulant and other toxicological effects of veterinary and agricultural surfactants” (OTKA K109865) by the Hungarian Research Fund, and by the Momentum Program (Lendület) of the Hungarian Academy of Sciences.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Nanobiosensorics Group, Institute for Technical Physics and Material Science, Centre for Energy ResearchHungarian Academy of SciencesBudapestHungary
  2. 2.Agro-Environmental Research InstituteNational Agricultural Research and Innovation CentreBudapestHungary

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