Abstract
The bacterium designated strain, able to degrade ethylbenzene was isolated from petroleum contaminated soil at automobile workshops and petrol pumps in Tiruchirapalli, Tamil Nadu, India. Bacterial analysis of the samples revealed the presence of ethylbenzene degrading bacteria belonging to the genera Pseudomonas, Enterobacteriaceae, Moraxella, Bacillus and Micrococcus sp. The microbial consortium consists of SRA91 and SRA104 is having higher degradation of ethylbenzene. The biodegradation rate of ethylbenzene were relatively low in Bushnell Haas (BH) broth, but addition of carbon source had a substantial impact on the biodegradation of ethylbenzene, which suggested that carbon might provide a factor that was necessary for its ethylbenzene biodegradation. Influence of various carbon sources, incubation temperature and pH on ethylbenzene degradation from synthetic ethylbenzene-rich BH broth also studied. The results showed a rapid and efficient process of ethylbenzene degradation (99 %) from synthetic ethylbenzene-rich BH broth supplemented with glucose (1 %), inoculated by 1 % bacterial consortium (Enterobacteriaceae sp. SRA91 and SRA104) at incubation temperature of 45 °C at pH 7. This study suggests that isolated Enterobacteriaceae strains SRA91 and SRA104 may play an important role for biodegradation of ethylbenzene in the contaminated soil.
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Acknowledgement
This work was financially supported by the Department of Science and Technology (SERB/F/2765/2011-12) through Fast Track—Young Scientist Project scheme, Government of India.
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Ashok, S., Akila, V., Ayyasamy, P.M., Rajakumar, S. (2016). Bioremediation of Ethylbenzene by Soil Column Study and Bioreactor Study for Polluted Soil and Water Samples Using Optimized Bacterial Consortium. In: Prashanthi, M., Sundaram, R. (eds) Integrated Waste Management in India. Environmental Science and Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-27228-3_15
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