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Combining Single and Paired End RNA-seq Data for Differential Expression Analyses

  • Zhi-Ping Feng
  • Francois Collin
  • Terence P. SpeedEmail author
Conference paper
Part of the Abel Symposia book series (ABEL, volume 11)

Abstract

Combining RNA-seq data from different platforms should increase the power to detect differentially expressed genes, but may not be straightforward. Here we show how RUVs, a recently published method for removing unwanted variation and normalizing RNA-seq data, can combine the counts of single and paired end read libraries from formalin fixed, paraffin embedded tumor samples to permit differential expression analysis. Seven other intra- or inter-platform normalization methods are also described and the results are compared with those from RUVs.

Keywords

Differential Expression Analysis Volcano Plot Unwanted Variation Read Library Negative Control Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Dr. Johann Gagnon-Bartsch and Dr. Davide Risso for providing the latest version of the RUV packages and Prof. Gorden Smyth for the explanation of variances estimation in edgeR and voom-limma.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Zhi-Ping Feng
    • 1
  • Francois Collin
    • 2
  • Terence P. Speed
    • 1
    Email author
  1. 1.The Walter and Eliza Hall Institute of Medical ResearchParkvilleAustralia
  2. 2.Genomic Health, IncRedwood CityUSA

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