Abstract
Peroxidase positive granulocytes (neutrophils and macrophages) are identified by histochemical staining. This test is also referred to as Myeloperoxidase or Endtz test. It is performed when routine semen analysis shows that the number of round cells is ≥0.20 × 106/mL for male infertility patients. In the case of IUI or BASA samples, the cutoff for performing the Endtz test is ≥1.0 × 106/mL of semen. In this case, it is necessary to differentiate granulocytes such as neutrophils, polymorphonuclear leukocytes, and macrophages (primary sources of ROS, which can lead to male infertility) from germinal cells, all of which are seen as round cells. The procedure is done using suspended cells in a liquefied semen specimen and quantitated by counting stained cells using a Makler counting chamber.
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Agarwal, A., Gupta, S., Sharma, R. (2016). Leukocytospermia Quantitation (ENDTZ) Test. In: Agarwal, A., Gupta, S., Sharma, R. (eds) Andrological Evaluation of Male Infertility. Springer, Cham. https://doi.org/10.1007/978-3-319-26797-5_7
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DOI: https://doi.org/10.1007/978-3-319-26797-5_7
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