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Andrological Evaluation of Male Infertility pp 155–163Cite as

Reactive Oxygen Species (ROS) Measurement

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Abstract

Free oxygen radicals such as superoxide anion (O2¯), hydrogen peroxide (H2O2), hypochlorite (OHCl), and hydroxyl radical (OH) are called reactive oxygen species (ROS). Free radicals have a very short half-life and are continuously produced, mainly by white blood cells (WBC) and especially by granulocytes and abnormal sperm. Excessive amounts of ROS impair sperm quality. ROS levels can be measured with the chemiluminescence method using luminol as a probe.

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References

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Author information

Authors and Affiliations

  1. Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

    Ashok Agarwal PhD, HCLD (ABB), ELD (ACE), Sajal Gupta MD, MSc, TS (ABB) & Rakesh Sharma PhD

Authors
  1. Ashok Agarwal PhD, HCLD (ABB), ELD (ACE)
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  2. Sajal Gupta MD, MSc, TS (ABB)
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  3. Rakesh Sharma PhD
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Corresponding author

Correspondence to Ashok Agarwal PhD, HCLD (ABB), ELD (ACE) .

Editor information

Editors and Affiliations

  1. Cleveland Clinic, American Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Cleveland Clinic, American Center for Reproductive Medicine, CLEVELAND, Ohio, USA

    Sajal Gupta

  3. Cleveland Clinic, American Center for Reproductive Medicine, Cleveland, Ohio, USA

    Rakesh Sharma

Cleveland Clinic

Cleveland Clinic

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© 2016 Springer International Publishing Switzerland

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Agarwal, A., Gupta, S., Sharma, R. (2016). Reactive Oxygen Species (ROS) Measurement. In: Agarwal, A., Gupta, S., Sharma, R. (eds) Andrological Evaluation of Male Infertility. Springer, Cham. https://doi.org/10.1007/978-3-319-26797-5_21

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  • DOI: https://doi.org/10.1007/978-3-319-26797-5_21

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-26795-1

  • Online ISBN: 978-3-319-26797-5

  • eBook Packages: MedicineMedicine (R0)

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