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Probing Cancer Cell Metabolism Using NMR Spectroscopy

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 899)

Abstract

Altered cellular metabolism is now accepted to be at the core of many diseases including cancer. Over the past 20 years, NMR has become a core technology to study these metabolic perturbations in detail. This chapter reviews current NMR-based methods for steady-state metabolism and, in particular, the use of non-radioactive stable isotope-enriched tracers. Opportunities and challenges for each method, such as 1D 1H NMR spectroscopy and 13C carbon-based NMR spectroscopic methods, are discussed. Ultimately, the combination of NMR and mass spectra as orthogonal technologies are required to compensate for the drawbacks of each technique when used singly are discussed.

Keywords

NMR Cancer Metabolism 131Metabolomics Tracer analysis Stable isotopes HSQC MS 

Notes

Acknowledgements

The authors thank BBSRC and AstraZeneca for supporting Kate Hollinshead with a CASE studentship, the Wellcome Trust for supporting the HWB-NMR facility in Birmingham and lastly Sylvia C Miller and Jay Nath for critical reading of the manuscript.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Institute of Metabolism and Systems ResearchUniversity of BirminghamBirminghamUK

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