Quantification of Lung Metastases from In Vivo Mouse Models

  • Joan Chang
  • Janine T. ErlerEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 899)


Cancer research has made significant progress in terms of understanding and targeting primary tumors; however, the challenge remains for the successful treatment of metastatic cancers. This highlights the importance to use in vivo models to study the metastatic process, as well as for preclinical testing of compounds that could inhibit metastasis. As a result, proper quantification of metastases from in vivo models is of the utmost significance. Here, we provide a detailed protocol for collecting and handling lung tissues from mice, and guidance for subsequent analysis of metastases, as well as interpretation of data.


Quantification Metastasis In vivo model Tissue collection ImageJ 


  1. 1.
    Sporn MB. The war on cancer. Lancet. 1996;347:1377–81.CrossRefPubMedGoogle Scholar
  2. 2.
    Chang J, Erler J. Hypoxia-mediated metastasis. Adv Exp Med Biol. 2014;772:55–81Google Scholar
  3. 3.
    Khanna C, Hunter K. Modeling metastasis in vivo. Carcinogenesis. 2005;26:513–23.CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Biotech Research & Innovation Centre (BRIC)University of CopenhagenCopenhagenDenmark
  2. 2.Department of Cell Biology and PhysiologyUniversity of North Carolina at Chapel HillChapel HillUSA

Personalised recommendations