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Lipids in Plant and Algae Development pp 159–175Cite as

Chemical Genetics in Dissecting Membrane Glycerolipid Functions

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Part of the book series: Subcellular Biochemistry ((SCBI,volume 86))

Abstract

Chemical genetics has emerged as a powerful approach to dissect biological processes, based on the utilization of small molecules disturbing the function of specific target proteins. By analogy with classical genetics, ‘reverse chemical genetics’ refers to the utilization of drugs acting on a known target, enabling its functional characterization at the levels of the cells, tissues and organisms. Likewise, ‘direct chemical genetics’ refers to the utilization of a drug of unknown mode of action, but triggering a phenotype of interest. In that case, one has to identify the target(s) possibly blocked (or possibly activated) by the small molecule. This chapter illustrates both approaches, like the analysis of the elongation of fatty acids, the biosynthesis of galactoglycerolipids or the catabolism of phosphoglycerolipids by reverse chemical genetics or the study of the membrane glycerolipid remodeling triggered upon phosphate starvation, by direct chemical genetics.

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Acknowledgements

All authors are supported by a grant from Agence Nationale de la Recherche (ANR Reglisse). EM is also supported by the ANR DiaDomOil grant, the OCEANOMICS program from the French Ministry of Research and the Institut Carnot LISA (Lipides pour la santé et l’industrie).

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Authors and Affiliations

  1. UMR 5168 CNRS-CEA-INRA-Université Grenoble Alpes, Laboratoire de Physiologie Cellulaire et Végétale, iRTSV, CEA-Grenoble, Grenoble, France

    Florian Chevalier & Eric Maréchal

  2. UMR 7265 CNRS-CEA-Université Aix-Marseille, Biologie Végétale et Microbiologie Environnementale, iBEB, CEA Cadarache, Saint-Paul-lez-Durance, France

    Laura Cuyàs Carrera & Laurent Nussaume

Authors
  1. Florian Chevalier
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  2. Laura Cuyàs Carrera
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  3. Laurent Nussaume
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  4. Eric Maréchal
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Corresponding author

Correspondence to Eric Maréchal .

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Editors and Affiliations

  1. Academia Sinica, Institute of Plant and Microbial Bi, Taipei, Taiwan

    Yuki Nakamura

  2. Institut de Biologie Environnementale et, Université Aix Marseille, Saint-Paul-lez-Durance, Bouches-du-Rhône, France

    Yonghua Li-Beisson

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Chevalier, F., Carrera, L.C., Nussaume, L., Maréchal, E. (2016). Chemical Genetics in Dissecting Membrane Glycerolipid Functions. In: Nakamura, Y., Li-Beisson, Y. (eds) Lipids in Plant and Algae Development. Subcellular Biochemistry, vol 86. Springer, Cham. https://doi.org/10.1007/978-3-319-25979-6_7

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