Omics in Chlamydomonas for Biofuel Production

  • Hanna R. Aucoin
  • Joseph Gardner
  • Nanette R. Boyle
Part of the Subcellular Biochemistry book series (SCBI, volume 86)


In response to demands for sustainable domestic fuel sources, research into biofuels has become increasingly important. Many challenges face biofuels in their effort to replace petroleum fuels, but rational strain engineering of algae and photosynthetic organisms offers a great deal of promise. For decades, mutations and stress responses in photosynthetic microbiota were seen to result in production of exciting high-energy fuel molecules, giving hope but minor capability for design. However, ‘-omics’ techniques for visualizing entire cell processing has clarified biosynthesis and regulatory networks. Investigation into the promising production behaviors of the model organism C. reinhardtii and its mutants with these powerful techniques has improved predictability and understanding of the diverse, complex interactions within photosynthetic organisms. This new equipment has created an exciting new frontier for high-throughput, predictable engineering of photosynthetically produced carbon-neutral biofuels.


Nutrient limitation Triacylglycerols Biohydrogen Stress response Gene discovery 




Acetyl-CoA carboxylase, E.C.


Non-specific protein-tyrosine kinase, E.C.


(Acetyl-CoA carboxylase) kinase 2, E.C.


Alcohol dehydrogenase 1/Formaldehyde dehydrogenase (FDH1), E.C.


Alcohol dehydrogenase (NADP+), E.C.


Alcohol dehydrogenase Isoform, E.C.

ADP-Glc PPase

ADP-glucose pyrophosphorylase, E.C.


Diacylglycerol acyltransferase, E.C.


Fatty acid synthase, E.C.


Glycerol-3-phosphate acyltransferase, E.C.


Ferredoxin hydrogenase, E.C.


Ferredoxin hydrogenase Isoform, E.C.


Isocitrate lyase, E.C.


Lactate dehydrogenase, E.C.


Lysophosphatidic acid acyltransferase, E.C.


Malonyl acyl carrier protein transferase, E.C.


Major lipid droplet protein


Phosphate acetyltransferase, E.C.


Phosphate acetyltransferase Isoform, E.C.


Pyruvate decarboxylase, E.C.


Phospholipid diacylglycerol acyltransferase, E.C.


Pyruvate dehydrogenase, E.C.


Formate C-acetyltransferase, E.C.



Diacylglycerol acyltransferase


Diacylglycerol acyltransferase


Glycerol-3-phosphate dehydrogenase


Glycerol-3-phosphate dehydrogenase


Light Harvesting Complex Chlorophyll a-b binding protein


Light Harvesting Complex stress-related 2


Lysophosphatidic acid acyltransferase


Major lipid droplet protein


Phospholipid diacylglycerol acyltransferase





Fatty acid


Ferredoxin (oxidized)


Ferredoxin (reduced)




Pyruvate ferredoxin (oxidized)


Pyruvate ferredoxin (reduced)


Sulfoquinovovosyl diacylglycerol



Structures and Proteins


Cytochrome b6 (small subunit of cytochrome b6f complex)


Cytochrome f (large subunit of cytochrome b6f complex)




Ferredoxin – NADP(+) reductase


Light Harvesting Complex


Plastidial NAD(P)H dehydrogenase complex


Chlorophyll a P680 (680 nm)


Chlorophyll a P700 (700 nm)




Plastoquinone (reduced)


Photosystem I


Photosystem II


Plastoquinone (oxidized)



2-Dimensional Gel Electrophoresis


Gas Chromatography/Mass Spectroscopy


Heat Shock


Liquid Chromatography/Mass Spectroscopy


Matrix-Assisted Laser Desorption/Ionization


Nuclear Magnetic Resonance


Metabolite Flux Analysis


Time of Flight


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Hanna R. Aucoin
    • 1
  • Joseph Gardner
    • 1
  • Nanette R. Boyle
    • 1
  1. 1.Department of Chemical and Biological EngineeringColorado School of MinesGoldenUSA

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