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How to Take a Biopsy

  • Marcel F. JonkmanEmail author
  • Gilles F. H. Diercks
Chapter

Abstract

The skin is an organ that is easy to sample for microscopy. Sections of the skin may reveal the split level of the blister, the type and distribution of invading inflammatory cells, and the presence, class, and distribution pattern of autoantibodies. For this, one needs to take a biopsy from a location with smallest change of sample error and specially fixate and the transport the specimen for the requested microscopic technique. All together, this is the playing ground of the dermatologist. Due to special transport requirements, dermatologists even take conjunctiva biopsies for immunofluorescence (IF) in case of suspicion of an autoimmune bullous disease of the eye.

Keywords

Histopathology Immunofluorescence Biopsy 

References

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Additional Reading

  1. Powell AM, Black M. A stepwise approach to the diagnosis of blisters in the clinic. Clin Dermatol. 2001;19:598–606.CrossRefPubMedGoogle Scholar

On the Web

  1. Levitt J, Bernardo S, Whang T. Videos in clinical medicine. How to perform a punch biopsy of the skin. N Engl J Med. 2013;369:e13. http://www.nejm.org/doi/full/10.1056/NEJMvcm1105849. CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Department of DermatologyCenter for Blistering Diseases, University Medical Center Groningen, University of GroningenGroningenthe Netherlands
  2. 2.Departments of Pathology and Medical BiologyCenter for Blistering Diseases, University Medical Center Groningen, University of GroningenGroningenthe Netherlands

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