Visualizing Cortical Tissue Optical Changes During Seizure Activity with Optical Coherence Tomography

  • M. M. EberleEmail author
  • C. L. Rodriguez
  • J. I. Szu
  • Y. Wang
  • M. S. Hsu
  • D. K. Binder
  • B. H. Park
Part of the Computational Biology book series (COBO, volume 22)


Optical coherence tomography (OCT) is a label-free, high resolution, minimally invasive imaging tool, which can produce millimeter depth-resolved cross-sectional images. We identified changes in the backscattered intensity of infrared light, which occurred during the development of induced seizures in vivo in mice. In a large region of interest, we observed significant decreases in the OCT intensity from cerebral cortex tissue preceding and during generalized tonic-clonic seizures induced with pentylenetetrazol (PTZ). We then leveraged the full spatiotemporal resolution of OCT by studying the temporal evolution of localized changes in backscattered intensity in three dimensions and analyzed the seizure propagation in time-resolved 3D functional images. This allowed for a better understanding and visualization of this biological phenomenon.


Optical Coherence Tomography Maximum Intensity Projection Imaging Depth Visualization Technique Cortical Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was sponsored at UC Riverside by the National Institutes of Health R00-EB007241, K08-NS059674, and R01-NS081243; the National Science Foundation IGERT Video Bioinformatics DGE 0903667; and the UC Discovery Grant #213073.


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • M. M. Eberle
    • 1
    Email author
  • C. L. Rodriguez
    • 1
  • J. I. Szu
    • 2
  • Y. Wang
    • 1
  • M. S. Hsu
    • 3
  • D. K. Binder
    • 4
  • B. H. Park
    • 1
  1. 1.Department of Bioengineering, Materials Science and Engineering 243University of CaliforniaRiversideUSA
  2. 2.Translational Neuroscience LaboratoryUniversity of CaliforniaRiversideUSA
  3. 3.Translational Neuroscience LaboratoryUniversity of CaliforniaRiversideUSA
  4. 4.School of MedicineUniversity of CaliforniaRiversideUSA

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