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Investigating Bacterial Protein Synthesis Using Systems Biology Approaches

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Prokaryotic Systems Biology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 883))

Abstract

Protein synthesis is essential for bacterial growth and survival. Its study in Escherichia coli helped uncover features conserved among bacteria as well as universally. The pattern of discovery and the identification of some of the longest-known components of the protein synthesis machinery, including the ribosome itself, tRNAs, and translation factors proceeded through many stages of successively more refined biochemical purifications, finally culminating in the isolation to homogeneity, identification, and mapping of the smallest unit required for performing the given function. These early studies produced a wealth of information. However, many unknowns remained. Systems biology approaches provide an opportunity to investigate protein synthesis from a global perspective, overcoming the limitations of earlier ad hoc methods to gain unprecedented insights. This chapter reviews innovative systems biology approaches, with an emphasis on those designed specifically for investigating the protein synthesis machinery in E. coli.

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Authors and Affiliations

  1. Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada, M5S 1A8

    Alla Gagarinova & Andrew Emili

  2. Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada, M5S 3E1

    Alla Gagarinova & Andrew Emili

Authors
  1. Alla Gagarinova
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  2. Andrew Emili
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Correspondence to Alla Gagarinova .

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Editors and Affiliations

  1. Cellular and Molecular Pharmacology, Univ of California, San Francisco, SAN FRANCISCO, California, USA

    Nevan J. Krogan, PhD

  2. Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada

    Mohan Babu, PhD

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Gagarinova, A., Emili, A. (2015). Investigating Bacterial Protein Synthesis Using Systems Biology Approaches. In: Krogan, PhD, N., Babu, PhD, M. (eds) Prokaryotic Systems Biology. Advances in Experimental Medicine and Biology, vol 883. Springer, Cham. https://doi.org/10.1007/978-3-319-23603-2_2

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