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Genetic, Biochemical, and Structural Analyses of Bacterial Surface Polysaccharides

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Prokaryotic Systems Biology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 883))

Abstract

Surface polysaccharides are an often essential component of the outer surface of bacteria. They may serve to protect organisms from harsh environmental conditions and to increase virulence. The focus of this review will be to introduce polysaccharide biosynthesis and export from the cell, and the associated techniques used to determine these glycostructures. Protein interactions and proteomics will then be discussed while introducing systems biology approaches used to determine protein–protein and protein–polysaccharide interactions. The final section will address related screening methods used to study gene regulation in bacteria relating to polysaccharide gene clusters and their associated regulators. The goal of this review will be to highlight key studies that have increased our knowledge of glycobiology and discuss novel methods that examine this field at the cellular level using systems biology.

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Acknowledgements

We thank Chris Whitfield, Bradley Clarke, and other members of the Whitfield lab for helpful discussions during the writing of this review.

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Authors and Affiliations

  1. Agriculture and Food Laboratory, Laboratory Services, University of Guelph, 95 Stone Rd. W., Guelph, ON, N1H 8J7, Canada

    Colin A. Cooper

  2. Department of Molecular and Cellular Biology, University of Guelph, 95 Stone Road, Guelph, ON, N1H 8J7, Canada

    Iain L. Mainprize & Nicholas N. Nickerson

  3. Department of Infectious Diseases, Genentech Inc., South San Francisco, CA, 94080, USA

    Nicholas N. Nickerson

Authors
  1. Colin A. Cooper
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  2. Iain L. Mainprize
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  3. Nicholas N. Nickerson
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Correspondence to Colin A. Cooper .

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Editors and Affiliations

  1. Cellular and Molecular Pharmacology, Univ of California, San Francisco, SAN FRANCISCO, California, USA

    Nevan J. Krogan, PhD

  2. Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada

    Mohan Babu, PhD

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Cooper, C.A., Mainprize, I.L., Nickerson, N.N. (2015). Genetic, Biochemical, and Structural Analyses of Bacterial Surface Polysaccharides. In: Krogan, PhD, N., Babu, PhD, M. (eds) Prokaryotic Systems Biology. Advances in Experimental Medicine and Biology, vol 883. Springer, Cham. https://doi.org/10.1007/978-3-319-23603-2_16

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