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Beneficial Microorganisms in Medical and Health Applications pp 1–18Cite as

Health Effects of Pro- and Prebiotics: Utilization of Sophisticated In Vitro Tools

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Part of the book series: Microbiology Monographs ((MICROMONO,volume 28))

Abstract

This chapter is a summary of my keynote lecture given during the International Conference on Beneficial Microbes in Penang, Malaysia, from 27 to 29 May 2014. It describes the use of sophisticated dynamic, computer-controlled in vitro models of the gastrointestinal (GI) tract, developed by the Dutch Organization for Applied Scientific Research (TNO), nicknamed TIM. Among others, these have been used for determining and predicting survival of probiotics and the effects of prebiotics on the composition and activity of the gut microbiota. These sophisticated multicompartmental models closely mimic the dynamic conditions in the gastrointestinal (GI) tract and are therefore perfect tools to study mechanistically what happens in the GI tract. They can be used to study and optimize survival of probiotic strains and to screen for, e.g., an efficacious dose of prebiotics. Some examples on their use in optimizing probiotic survival, for instance, by combining them with prebiotics or developing a protective coating, are given. Also, the use of stable isotope-labeled substrates to trace metabolism of the gut microbiota as a tool to decipher what is going on in the colon is highlighted. Specific labeling of members of the microbiota and cross feeding are shown.

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  1. Beneficial Microbes Consultancy, Wageningen, The Netherlands

    Koen Venema

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  1. Universiti Sains Malaysia, School of Industrial Technology, Penang, Malaysia

    Min-Tze Liong

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Venema, K. (2015). Health Effects of Pro- and Prebiotics: Utilization of Sophisticated In Vitro Tools. In: Liong, MT. (eds) Beneficial Microorganisms in Medical and Health Applications. Microbiology Monographs, vol 28. Springer, Cham. https://doi.org/10.1007/978-3-319-23213-3_1

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