Automated Motion Analysis of Adherent Cells in Monolayer Culture
This paper presents a novel method for tracking and characterizing adherent cells in monolayer culture. A system of cell tracking employing computer vision techniques was applied to time-lapse videos of replicate normal human uro-epithelial cell cultures exposed to different concentrations of adenosine triphosphate (ATP), acquired over a 20 h period. Subsequent analysis, comprising feature extraction, demonstrated the ability of the technique to successfully separate the modulated classes of cell.
KeywordsInterstitial Cystitis Urothelial Cell Distance Image Migration Speed Computer Vision Technique
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