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Analytical Methodology for Lipid Extraction and Quantification from Oleaginous Microorganisms

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Modern Techniques and Solvents for the Extraction of Microbial Oils

Part of the book series: SpringerBriefs in Molecular Science ((GREENCHEMIST))

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Abstract

This chapter reviews the development of extraction and analytical techniques for lipids from microorganisms. The commonly used extraction techniques of total lipid such as «Folch», «Bligh and Dyer», and Soxhlet Extraction methods are detailed and explained. A special focus has been made for the complementary analytical methods such as HP-TLC (High Performance Thin Layer Chromatography) which gives details about the repartition of the different classes of lipids and GC (Gas Chromatography) which brings quantitative analysis of all fatty acids chains present in the lipid microbial extract.

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Correspondence to Farid Chemat .

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Meullemiestre, A., Breil, C., Abert-Vian, M., Chemat, F. (2015). Analytical Methodology for Lipid Extraction and Quantification from Oleaginous Microorganisms. In: Modern Techniques and Solvents for the Extraction of Microbial Oils. SpringerBriefs in Molecular Science(). Springer, Cham. https://doi.org/10.1007/978-3-319-22717-7_2

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