Abstract
Electrospinning is a technique used in the production of polymer nanofibre meshes. The use of biodegradable and biocompatible polymers to produce nanofibres that closely mimic the extracellular matrix (ECM) of different tissues has opened a wide range of possibilities for the application of electrospinning in Tissue Engineering. It is believed that nano-features (such as voids and surface cues) present in nanofibre mesh scaffolds, combined with the chemical composition of the fibres, can stimulate cell attachment, growth and differentiation. Despite the widespread use of electrospun nanofibres in tissue engineering, the present chapter will focus on the advances made in the utilisation of these materials in bone, cartilage and tooth related applications. Several aspects will be taken into consideration, namely the choice of polymers, the surface modification of the nanofibres in order to achieve mineralisation, and also the biological application of such materials.
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References
Abdal-hay A, Hwang M-G, Lim JK (2012) In vitro bioactivity of titanium implants coated with bicomponent hybrid biodegradable polymers. J Sol-Gel Sci Technol 64:756–764
Aghdam RM, Najarian S, Shakhesi S, Khanlari S, Shaabani K, Sharifi S (2012) Investigating the effect of PGA on physical and mechanical properties of electrospun PCL/PGA blend nanofibers. J Appl Polym Sci 124:123–131
Ajalloueian F, Zeiai S, Fossum M, Hilborn JG (2014a) Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue expansion. Biomaterials 35:5741–5748
Ajalloueian F, Tavanai H, Hilborn J, Donzel-Gargand O, Leifer K, Wickham A et al (2014b) Emulsion electrospinning as an approach to fabricate PLGA/chitosan nanofibers for biomedical applications. BioMed Res Int 2014:475280
Albuquerque MT, Valera MC, Nakashima M, Nor JE, Bottino MC (2014) Tissue-engineering-based strategies for regenerative endodontics. J Dent Res 93:1222–1231
Allo BA, Rizkalla AS, Mequanint K (2010) Synthesis and electrospinning of epsilon-polycaprolactone-bioactive glass hybrid biomaterials via a sol-gel process. Langmuir 26:18340–18348
Amoroso NJ, D’Amore A, Hong Y, Rivera CP, Sacks MS, Wagner WR (2012) Microstructural manipulation of electrospun scaffolds for specific bending stiffness for heart valve tissue engineering. Acta Biomater 8:4268–4277
Andiappan M, Sundaramoorthy S, Panda N, Meiyazhaban G, Winfred S, Venkataraman G et al (2013) Electrospun eri silk fibroin scaffold coated with hydroxyapatite for bone tissue engineering applications. Prog Biomater 2:1–11
Aniket RR, Hall B, Marriott I, El-Ghannam A (2014) Early osteoblast responses to orthopedic implants: synergy of surface roughness and chemistry of bioactive ceramic coating. J Biomed Mater Res Part A 103:1961–1973
Anton F (1934) Process and apparatus for preparing artificial threads. Google Patents
Anton F (1944) Method and apparatus for spinning. Google Patents
Araujo JV, Martins A, Leonor IB, Pinho ED, Reis RL, Neves NM (2008) Surface controlled biomimetic coating of polycaprolactone nanofiber meshes to be used as bone extracellular matrix analogues. J Biomater Sci Polym Ed 19:1261–1278
Araujo JV, Cunha-Reis C, Rada T, da Silva MA, Gomes ME, Yang Y et al (2010) Dynamic culture of osteogenic cells in biomimetically coated poly(caprolactone) nanofibre mesh constructs. Tissue Eng Part A 16:557–563
Asgharnia S, Alizadeh P (2013) Synthesis and characterization of SiO2–CaO–P2O5–MgO based bioactive glass and glass-ceramic nanofibres by electrospinning. Mater Lett 101:107–110
Badami AS, Kreke MR, Thompson MS, Riffle JS, Goldstein AS (2006) Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates. Biomaterials 27:596–606
Badylak SF, Taylor D, Uygun K (2011) Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 13:27–53
Baker BM, Mauck RL (2007) The effect of nanofiber alignment on the maturation of engineered meniscus constructs. Biomaterials 28:1967–1977
Baykan E, Koc A, Eser Elcin A, Murat Elcin Y (2014) Evaluation of a biomimetic poly(ε-caprolactone)/β-tricalcium phosphate multispiral scaffold for bone tissue engineering: in vitro and in vivo studies. Biointerphases 9:029011
Bianco A, Di Federico E, Cacciotti I (2011) Electrospun poly(ε-caprolactone)-based composites using synthesized β-tricalcium phosphate. Polym Adv Technol 22:1832–1841
Bohner M, Lemaitre J (2009) Can bioactivity be tested in vitro with SBF solution? Biomaterials 30:2175–2179
Boland ED, Wnek GE, Simpson DG, Pawlowski KJ, Bowlin GL (2001) Tailoring tissue engineering scaffolds using electrostatic processing techniques: a study of poly(glycolic acid) electrospinning. J Macromol Sci-Pure Appl Chem 38:1231–1243
Boland ED, Telemeco TA, Simpson DG, Wnek GE, Bowlin GL (2004) Utilizing acid pretreatment and electrospinning to improve biocompatibility of poly(glycolic acid) for tissue engineering. J Biomed Mater Res B Appl Biomater 71B:144–152
Bottino MC, Kamocki K, Yassen GH, Platt JA, Vail MM, Ehrlich Y et al (2013) Bioactive nanofibrous scaffolds for regenerative endodontics. J Dent Res 92:963–969
Braunecker J, Baba M, Milroy GE, Cameron RE (2004) The effects of molecular weight and porosity on the degradation and drug release from polyglycolide. Int J Pharm 282:19–34
Brenner EK, Schiffman JD, Thompson EA, Toth LJ, Schauer CL (2012) Electrospinning of hyaluronic acid nanofibers from aqueous ammonium solutions. Carbohydr Polym 87:926–929
Brenner EK, Schiffman JD, Toth LJ, Szewczyk JC, Schauer CL (2013) Phosphate salts facilitate the electrospinning of hyaluronic acid fiber mats. J Mater Sci 48:7805–7811
Bueno EM, Glowacki J (2009) Cell-free and cell-based approaches for bone regeneration. Nat Rev Rheumatol 5:685–697
Buschmann J, Harter L, Gao S, Hemmi S, Welti M, Hild N et al (2012) Tissue engineered bone grafts based on biomimetic nanocomposite PLGA/amorphous calcium phosphate scaffold and human adipose-derived stem cells. Injury 43:1689–1697
Cai S, Xu H, Jiang Q, Yang Y (2013) Novel 3D electrospun scaffolds with fibers oriented randomly and evenly in three dimensions to closely mimic the unique architectures of extracellular matrices in soft tissues: fabrication and mechanism study. Langmuir 29:2311–2318
Casper CL, Yamaguchi N, Kiick KL, Rabolt JF (2005) Functionalizing electrospun fibers with biologically relevant macromolecules. Biomacromolecules 6:1998–2007
Cestari M, Muller V, da Silva Rodrigues JH, Nakamura CV, Rubira AF, Muniz EC (2014) Preparing silk fibroin nanofibers through electrospinning: further heparin immobilization toward hemocompatibility improvement. Biomacromolecules 15:1762–1767
Chakrapani VY, Gnanamani A, Giridev VR, Madhusoothanan M, Sekaran G (2012) Electrospinning of type I collagen and PCL nanofibers using acetic acid. J Appl Polym Sci 125:3221–3227
Chen Z, Mo X, He C, Wang H (2008) Intermolecular interactions in electrospun collagen-chitosan complex nanofibers. Carbohydr Polym 72:410–418
Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ (2010) Electrospun collagen-chitosan nanofiber: a biomimetic extracellular matrix for endothelial cell and smooth muscle cell. Acta Biomater 6:372–382
Cho HJ, Yoo YJ, Kim JW, Park YH, Bae DG, Um IC (2012) Effect of molecular weight and storage time on the wet- and electro-spinning of regenerated silk fibroin. Polym Degrad Stab 97:1060–1066
Choi JS, Lee SJ, Christ GJ, Atala A, Yoo JJ (2008) The influence of electrospun aligned poly(epsilon-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials 29:2899–2906
Cui W, Li X, Xie C, Zhuang H, Zhou S, Weng J (2010) Hydroxyapatite nucleation and growth mechanism on electrospun fibers functionalized with different chemical groups and their combinations. Biomaterials 31:4620–4629
Cui Z, Wright LD, Guzzo R, Freeman JW, Drissi HD, Nair LS (2013) Poly (d-lactide)/poly (caprolactone) nanofiber-thermogelling chitosan gel composite scaffolds for osteochondral tissue regeneration in a rat model. J Bioact Compat Pol :0883911512472278
Dai X, Shivkumar S (2007a) Electrospinning of PVA-calcium phosphate sol precursors for the production of fibrous hydroxyapatite. J Am Ceram Soc 90:1412–1419
Dai X, Shivkumar S (2007b) Electrospinning of hydroxyapatite fibrous mats. Mater Lett 61:2735–2738
Daranarong D, Thapsukhon B, Swanandy N, Molloy R, Punyodom W, Foster LJR (2014) Application of low loading of collagen in electrospun poly (L-lactide)-co-(epsilon-caprolactone) nanofibrous scaffolds to promote cellular biocompatibility. Polym Int 63:1254–1262
De Vrieze S, Westbroek P, Van Camp T, Van Langenhove L (2007) Electrospinning of chitosan nanofibrous structures: feasibility study. J Mater Sci 42:8029–8034
Deng M, Kumbar SG, Nair LS, Weikel AL, Allcock HR, Laurencin CT (2011) Biomimetic structures: biological implications of dipeptide-substituted polyphosphazene–polyester blend nanofiber matrices for load-bearing bone regeneration. Adv Funct Mater 21:2641–2651
Duan B, Yuan X, Zhu Y, Zhang Y, Li X, Zhang Y et al (2006) A nanofibrous composite membrane of PLGA-chitosan/PVA prepared by electrospinning. Eur Polym J 42:2013–2022
Ducheyne P, Hench LL, Kagan A, Martens M, Mulier JC (1979) Short-term bonding behavior of bioglass coatings on metal-substrate. Arch Orthop Trauma Surg 94:155–160
el Kenawy R, Bowlin GL, Mansfield K, Layman J, Simpson DG, Sanders EH et al (2002) Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. J Control Release 81:57–64
Erisken C, Kalyon DM, Wang H, Ornek-Ballanco C, Xu J (2011) Osteochondral tissue formation through adipose-derived stromal cell differentiation on biomimetic polycaprolactone nanofibrous scaffolds with graded insulin and beta-glycerophosphate concentrations. Tissue Eng A 17:1239–1252
Flemming RG, Murphy CJ, Abrams GA, Goodman SL, Nealey PF (1999) Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials 20:573–588
Frohbergh ME, Katsman A, Botta GP, Lazarovici P, Schauer CL, Wegst UG et al (2012) Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering. Biomaterials 33:9167–9178
Gamble JL, Harvard Medical S (1941) Chemical anatomy, physiology and pathology of extracellular fluid: a lecture syllabus. Dept. of Pediatrics, The Harvard Medical School, Cambridge
Gao C, Gao Q, Bao X, Li Y, Teramoto A, Abe K (2011) Preparation and in vitro bioactivity of novel mesoporous borosilicate bioactive glass nanofibers. J Am Ceram Soc 94:2841–2845
Gao CX, Gao Q, Li YD, Rahaman MN, Teramoto A, Abe K (2013) In vitro evaluation of electrospun gelatin-bioactive glass hybrid scaffolds for bone regeneration. J Appl Polym Sci 127:2588–2599
Geng X, Kwon O-H, Jang J (2005) Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials 26:5427–5432
Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani M-H, Ramakrishna S (2008) Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29:4532–4539
Gopal R, Kaur S, Ma Z, Chan C, Ramakrishna S, Matsuura T (2006) Electrospun nanofibrous filtration membrane. J Membr Sci 281:581–586
Green AM, Jansen JA, van der Waerden JP, von Recum AF (1994) Fibroblast response to microtextured silicone surfaces: texture orientation into or out of the surface. J Biomed Mater Res 28:647–653
He J, Cheng Y, Li P, Zhang Y, Zhang H, Cui S (2013) Preparation and characterization of biomimetic tussah silk fibroin/chitosan composite nanofibers. Iran Polym J 22:537–547
Hench LL, Paschall HA (1973) Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. J Biomed Mater Res 7:25–42
Hench LL, Pantano CG, Buscemi PJ, Greenspan DC (1977) Analysis of bioglass fixation of hip prostheses. J Biomed Mater Res 11:267–282
Homayoni H, Ravandi SAH, Valizadeh M (2009) Electrospinning of chitosan nanofibers: processing optimization. Carbohydr Polym 77:656–661
Hong S, Kim G (2011) Fabrication of size-controlled three-dimensional structures consisting of electrohydrodynamically produced polycaprolactone micro/nanofibers. Appl Phys A 103:1009–1014
Hong Y, Chen X, Jing X, Fan H, Guo B, Gu Z et al (2010) Preparation, bioactivity, and drug release of hierarchical nanoporous bioactive glass ultrathin fibers. Adv Mater 22:754–758
Hsu SH, Whu SW, Hsieh SC, Tsai CL, Chen DC, Tan TS (2004) Evaluation of chitosan-alginate-hyaluronate complexes modified by an RGD-containing protein as tissue-engineering scaffolds for cartilage regeneration. Artif Organs 28:693–703
Huang Z-M, Zhang YZ, Ramakrishna S, Lim CT (2004) Electrospinning and mechanical characterization of gelatin nanofibers. Polymer 45:5361–5368
Jamshidi Adegani F, Langroudi L, Ardeshirylajimi A, Dinarvand P, Dodel M, Doostmohammadi A et al (2014) Coating of electrospun poly(lactic-co-glycolic acid) nanofibers with willemite bioceramic: improvement of bone reconstruction in rat model. Cell Biol Int 38:1271–1279
Jeong H-G, Kim Y-E, Kim Y-J (2013) Fabrication of poly(vinyl acetate)/polysaccharide biocomposite nanofibrous membranes for tissue engineering. Macromol Res 21:1233–1240
Ji J, Bar-On B, Wagner HD (2012) Mechanics of electrospun collagen and hydroxyapatite/collagen nanofibers. J Mech Behav Biomed Mater 13:185–193
Jing X, Salick MR, Cordie T, Mi H-Y, Peng X-F, Turng L-S (2014) Electrospinning homogeneous nanofibrous poly(propylene carbonate)/gelatin composite scaffolds for tissue engineering. Ind Eng Chem Res 53:9391–9400
Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT (2008) Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg (Am Vol) 90A:36–42
Ki CS, Baek DH, Gang KD, Lee KH, Um IC, Park YH (2005) Characterization of gelatin nanofiber prepared from gelatin–formic acid solution. Polymer 46:5094–5102
Kim YB, Kim G (2012) Rapid-prototyped collagen scaffolds reinforced with PCL/[small beta]-TCP nanofibres to obtain high cell seeding efficiency and enhanced mechanical properties for bone tissue regeneration. J Mater Chem 22:16880–16889
Kim MS, Kim GH (2014) Highly porous electrospun 3D polycaprolactone/β-TCP biocomposites for tissue regeneration. Mater Lett 120:246–250
Kim HW, Lee HH, Knowles JC (2006a) Electrospinning biomedical nanocomposite fibers of hydroxyapatite/poly(lactic acid) for bone regeneration. J Biomed Mater Res A 79:643–649
Kim HW, Kim HE, Knowles JC (2006b) Production and potential of bioactive glass nanofibers as a next-generation biomaterial. Adv Funct Mater 16:1529–1535
Kim HW, Lee HH, Chun GS (2008) Bioactivity and osteoblast responses of novel biomedical nanocomposites of bioactive glass nanofiber filled poly(lactic acid). J Biomed Mater Res A 85:651–663
Kim SJ, Jang DH, Park WH, Min B-M (2010) Fabrication and characterization of 3-dimensional PLGA nanofiber/microfiber composite scaffolds. Polymer 51:1320–1327
Kim J-J, Bae W-J, Kim J-M, Kim J-J, Lee E-J, Kim H-W et al (2013) Mineralized polycaprolactone nanofibrous matrix for odontogenesis of human dental pulp cells. J Biomater Appl :0885328213495903
Kim H, Che L, Ha Y, Ryu W (2014a) Mechanically-reinforced electrospun composite silk fibroin nanofibers containing hydroxyapatite nanoparticles. Mater Sci Eng C 40:324–335
Kim MS, Son J, Lee H, Hwang H, Choi CH, Kim G (2014b) Highly porous 3D nanofibrous scaffolds processed with an electrospinning/laser process. Curr Appl Phys 14:1–7
Kokubo T (1991) Bioactive glass-ceramics – properties and applications. Biomaterials 12:155–163
Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–2915
Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T (1990) Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic. J Biomed Mater Res 24:721–734
Kolbuk D, Sajkiewicz P, Maniura-Weber K, Fortunato G (2013) Structure and morphology of electrospun polycaprolactone/gelatine nanofibres. Eur Polym J 49:2052–2061
Krucinska I, Chrzanowska O, Bogun M, Kowalczuk M, Dobrzynski P (2014) Fabrication of PLGA/HAP and PLGA/PHB/HAP fibrous nanocomposite materials for osseous tissue regeneration. Autex Res J 14:95–110
Kumbar SG, Nukavarapu SP, James R, Nair LS, Laurencin CT (2008) Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials 29:4100–4107
Lai G-J, Shalumon KT, Chen S-H, Chen J-P (2014) Composite chitosan/silk fibroin nanofibers for modulation of osteogenic differentiation and proliferation of human mesenchymal stem cells. Carbohydr Polym 111:288–297
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920
Lao LH, Wang YJ, Zhu Y, Zhang YY, Gao CY (2011) Poly(lactide-co-glycolide)/hydroxyapatite nanofibrous scaffolds fabricated by electrospinning for bone tissue engineering. J Mater Sci Mater Med 22:1873–1884
Lee S, Obendorf SK (2007) Use of electrospun nanofiber web for protective textile materials as barriers to liquid penetration. Text Res J 77:696–702
Lee KH, Kim HY, Khil MS, Ra YM, Lee DR (2003) Characterization of nano-structured poly(ε-caprolactone) nonwoven mats via electrospinning. Polymer 44:1287–1294
Lee JH, Rim NG, Jung HS, Shin H (2010) Control of osteogenic differentiation and mineralization of human mesenchymal stem cells on composite nanofibers containing poly lactic-co-(glycolic acid) and hydroxyapatite. Macromol Biosci 10:173–182
Lee B-K, Ju YM, Cho J-G, Jackson JD, Lee SJ, Atala A et al (2012) End-to-side neurorrhaphy using an electrospun PCL/collagen nerve conduit for complex peripheral motor nerve regeneration. Biomaterials 33:9027–9036
Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK (2002) Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res 60:613–621
Li C, Vepari C, Jin H-J, Kim HJ, Kaplan DL (2006) Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27:3115–3124
Li X, Liu W, Sun L, Aifantis KE, Yu B, Fan Y et al (2014a) Resin composites reinforced by nanoscaled fibers or tubes for dental regeneration. BioMed Res Int 2014:13
Li D, Wu T, He N, Wang J, Chen W, He L et al (2014b) Three-dimensional polycaprolactone scaffold via needleless electrospinning promotes cell proliferation and infiltration. Colloids Surf B: Biointerfaces 121:432–443
Li G, Zhang T, Li M, Fu N, Fu Y, Ba K et al (2014c) Electrospun fibers for dental and craniofacial applications. Curr Stem Cell Res Ther 9:187–195
Lin HM, Lin YH, Hsu FY (2012) Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering. J Mater Sci Mater Med 23:2619–2630
Liu Y, Ma G, Fang D, Xu J, Zhang H, Nie J (2011) Effects of solution properties and electric field on the electrospinning of hyaluronic acid. Carbohydr Polym 83:1011–1015
Liverani L, Abbruzzese F, Mozetic P, Basoli F, Rainer A, Trombetta M (2014) Electrospinning of hydroxyapatite-chitosan nanofibers for tissue engineering applications. Asia Pac J Chem Eng 9:407–414
Lu L, Wu D, Zhang M, Zhou W (2012) Fabrication of polylactide/poly(ε-caprolactone) blend fibers by electrospinning: morphology and orientation. Ind Eng Chem Res 51:3682–3691
Lyu S, Huang C, Yang H, Zhang X (2013) Electrospun fibers as a scaffolding platform for bone tissue repair. J Orthop Res: Off Publ Orthop Res Soc 31:1382–1389
Ma Z, Chen F, Zhu YJ, Cui T, Liu XY (2011) Amorphous calcium phosphate/poly(D, L-lactic acid) composite nanofibers: electrospinning preparation and biomineralization. J Colloid Interface Sci 359:371–379
Martins A, Araujo JV, Reis RL, Neves NM (2007) Electrospun nanostructured scaffolds for tissue engineering applications. Nanomedicine 2:929–942
Martins A, Chung S, Pedro AJ, Sousa RA, Marques AP, Reis RL et al (2009) Hierarchical starch-based fibrous scaffold for bone tissue engineering applications. J Tissue Eng Regen Med 3:37–42
Martins A, Duarte AR, Faria S, Marques AP, Reis RL, Neves NM (2010) Osteogenic induction of hBMSCs by electrospun scaffolds with dexamethasone release functionality. Biomaterials 31:5875–5885
Matthews JA, Wnek GE, Simpson DG, Bowlin GL (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3:232–238
Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004a) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25:1289–1297
Min B-M, Lee SW, Lim JN, You Y, Lee TS, Kang PH et al (2004b) Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers. Polymer 45:7137–7142
Mo XM, Xu CY, Kotaki M, Ramakrishna S (2004) Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation. Biomaterials 25:1883–1890
Moffat KL, Wang IN, Rodeo SA, Lu HH (2009) Orthopedic interface tissue engineering for the biological fixation of soft tissue grafts. Clin Sports Med 28:157–176
Moroni L, Schotel R, Hamann D, de Wijn JR, van Blitterswijk CA (2008) 3D fiber-deposited electrospun integrated scaffolds enhance cartilage tissue formation. Adv Funct Mater 18:53–60
Murphy CM, Haugh MG, O’Brien FJ (2010) The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials 31:461–466
Nerurkar NL, Han W, Mauck RL, Elliott DM (2011) Homologous structure-function relationships between native fibrocartilage and tissue engineered from MSC-seeded nanofibrous scaffolds. Biomaterials 32:461–468
Nie H, Soh BW, Fu YC, Wang CH (2008) Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery. Biotechnol Bioeng 99:223–234
Novotna K, Zajdlova M, Suchy T, Hadraba D, Lopot F, Zaloudkova M et al (2014) Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells. J Biomed Mater Res A 102:3918–3930
Obata A, Ozasa H, Kasuga T, Jones JR (2013) Cotton wool-like poly(lactic acid)/vaterite composite scaffolds releasing soluble silica for bone tissue engineering. J Mater Sci Mater Med 24:1649–1658
Ohgo K, Zhao C, Kobayashi M, Asakura T (2003) Preparation of non-woven nanofibers of Bombyx mori silk, Samia cynthia ricini silk and recombinant hybrid silk with electrospinning method. Polymer 44:841–846
Olsson H, Petersson K, Rohlin M (2006) Formation of a hard tissue barrier after pulp cappings in humans. A systematic review. Int Endod J 39:429–442
Patlolla A, Arinzeh TL (2014) Evaluating apatite formation and osteogenic activity of electrospun composites for bone tissue engineering. Biotechnol Bioeng 111:1000–1017
Petersen W, Tillmann B (1998) Collagenous fibril texture of the human knee joint menisci. Anat Embryol 197:317–324
Poologasundarampillai G, Wang D, Li S, Nakamura J, Bradley R, Lee PD et al (2014) Cotton-wool-like bioactive glasses for bone regeneration. Acta Biomater 10:3733–3746
Powell HM, Supp DM, Boyce ST (2008) Influence of electrospun collagen on wound contraction of engineered skin substitutes. Biomaterials 29:834–843
Prabhakaran MP, Venugopal JR, Ramakrishna S (2009) Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substrates for nerve tissue engineering. Biomaterials 30:4996–5003
Qin X-H, Wang S-Y (2006) Filtration properties of electrospinning nanofibers. J Appl Polym Sci 102:1285–1290
Ramakrishna S, Jose R, Archana PS, Nair AS, Balamurugan R, Venugopal J et al (2010) Science and engineering of electrospun nanofibers for advances in clean energy, water filtration, and regenerative medicine. J Mater Sci 45:6283–6312
Recum AF, Shannon CE, Cannon CE, Long KJ, Kooten TG, Meyle J (1996) Surface roughness, porosity, and texture as modifiers of cellular adhesion. Tissue Eng 2:241–253
Rho KS, Jeong L, Lee G, Seo BM, Park YJ, Hong SD et al (2006) Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 27:1452–1461
Riboldi SA, Sampaolesi M, Neuenschwander P, Cossu G, Mantero S (2005) Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering. Biomaterials 26:4606–4615
Roosa SM, Kemppainen JM, Moffitt EN, Krebsbach PH, Hollister SJ (2010) The pore size of polycaprolactone scaffolds has limited influence on bone regeneration in an in vivo model. J Biomed Mater Res A 92:359–368
Sangsanoh P, Supaphol P (2006) Stability improvement of electrospun chitosan nanofibrous membranes in neutral or weak basic aqueous solutions. Biomacromolecules 7:2710–2714
Scarber RE, Salaam AD, Thomas V, Janowski GM, Dean D (2013) Direct sol-gel electrospinning of fibrous bioglass scaffolds for bone tissue engineering. J Biomater Tissue Eng 3:440–447
Schneider OD, Loher S, Brunner TJ, Uebersax L, Simonet M, Grass RN et al (2008a) Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells. J Biomed Mater Res B Appl Biomater 84B:350–362
Schneider OD, Loher S, Brunner TJ, Uebersax L, Simonet M, Grass RN et al (2008b) Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells. J Biomed Mater Res B Appl Biomater 84:350–362
Schneider OD, Weber F, Brunner TJ, Loher S, Ehrbar M, Schmidlin PR et al (2009) In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects. Acta Biomater 5:1775–1784
Schnell E, Klinkhammer K, Balzer S, Brook G, Klee D, Dalton P et al (2007) Guidance of glial cell. migration and axonal growth on electrospun nanofibers of poly-epsilon-caprolactone and a collagen/poly-epsilon-caprolactone blend. Biomaterials 28:3012–3025
Setton LA, Guilak F, Hsu EW, Vail TP (1999) Biomechanical factors in tissue engineered meniscal repair. Clin Orthop Relat Res 367S:S254–S272
Seyedjafari E, Soleimani M, Ghaemi N, Shabani I (2010) Nanohydroxyapatite-coated electrospun poly(L-lactide) nanofibers enhance osteogenic differentiation of stem cells and induce ectopic bone formation. Biomacromolecules 11:3118–3125
Sheikh FA, Ju HW, Moon BM, Park HJ, Kim JH, Lee OJ et al (2013) A novel approach to fabricate silk nanofibers containing hydroxyapatite nanoparticles using a three-way stopcock connector. Nanoscale Res Lett 8:15
Shin SH, Purevdorj O, Castano O, Planell JA, Kim HW (2012) A short review: recent advances in electrospinning for bone tissue regeneration. J Tissue Eng 3:2041731412443530
Silva CSR, Luz GM, Gamboa-martÍnez TC, Mano JF, GÓmez ribelles JL, GÓmez-tejedor JA (2013) Poly(ɛ-caprolactone) electrospun scaffolds filled with nanoparticles. Production and optimization according to Taguchi’s methodology. J Macromol Sci B 53:781–799
Sisson K, Zhang C, Farach-Carson MC, Chase DB, Rabolt JF (2010) Fiber diameters control osteoblastic cell migration and differentiation in electrospun gelatin. J Biomed Mater Res A 94A:1312–1320
Srouji S, Ben-David D, Lotan R, Livne E, Avrahami R, Zussman E (2011) Slow-release human recombinant bone morphogenetic protein-2 embedded within electrospun scaffolds for regeneration of bone defect: in vitro and in vivo evaluation. Tissue Eng A 17:269–277
Stanishevsky A, Chowdhury S, Chinoda P, Thomas V (2008) Hydroxyapatite nanoparticle loaded collagen fiber composites: microarchitecture and nanoindentation study. J Biomed Mater Res A 86A:873–882
Stankus JJ, Freytes DO, Badylak SF, Wagner WR (2008) Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix. J Biomater Sci Polym Ed 19:635–652
Su Y, Su Q, Liu W, Lim M, Venugopal JR, Mo X et al (2012) Controlled release of bone morphogenetic protein 2 and dexamethasone loaded in core-shell PLLACL-collagen fibers for use in bone tissue engineering. Acta Biomater 8:763–771
Teng S-H, Lee E-J, Wang P, Kim H-E (2008) Collagen/hydroxyapatite composite nanofibers by electrospinning. Mater Lett 62:3055–3058
Tetteh G, Khan AS, Delaine-Smith RM, Reilly GC, Rehman IU (2014) Electrospun polyurethane/hydroxyapatite bioactive scaffolds for bone tissue engineering: the role of solvent and hydroxyapatite particles. J Mech Behav Biomed Mater 39:95–110
Thorvaldsson A, Stenhamre H, Gatenholm P, Walkenstrom P (2008) Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules 9:1044–1049
Tiwari SK, Tzezana R, Zussman E, Venkatraman SS (2010) Optimizing partition-controlled drug release from electrospun core–shell fibers. Int J Pharm 392:209–217
Tuzlakoglu K, Bolgen N, Salgado AJ, Gomes ME, Piskin E, Reis RL (2005) Nano- and micro-fiber combined scaffolds: a new architecture for bone tissue engineering. J Mater Sci Mater Med 16:1099–1104
Van Lieshout MI, Vaz CM, Rutten MCM, Peters GWM, Baaijens FPT (2006) Electrospinning versus knitting: two scaffolds for tissue engineering of the aortic valve. J Biomater Sci Polym Ed 17:77–89
Vasita R, Katti DS (2006) Nanofibers and their applications in tissue engineering. Int J Nanomedicine 1:15–30
Venugopal JR, Zhang YZ, Ramakrishna S (2006) In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane. Artif Organs 30:440–446
Wang X, Grogan SP, Rieser F, Winkelmann V, Maquet V, Berge ML et al (2004) Tissue engineering of biphasic cartilage constructs using various biodegradable scaffolds: an in vitro study. Biomaterials 25:3681–3688
Wang C, Yan K-W, Lin Y-D, Hsieh PCH (2010) Biodegradable core/shell fibers by coaxial electrospinning: processing, fiber characterization, and its application in sustained drug release. Macromolecules 43:6389–6397
Wu Y, Hench LL, Du J, Choy K-L, Guo J (2004) Preparation of hydroxyapatite fibers by electrospinning technique. J Am Ceram Soc 87:1988–1991
Xia W, Zhang D, Chang J (2007) Fabrication and in vitro biomineralization of bioactive glass (BG) nanofibres. Nanotechnology 18:135601
Xin X, Hussain M, Mao JJ (2007) Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold. Biomaterials 28:316–325
Xu C, Yang F, Wang S, Ramakrishna S (2004) In vitro study of human vascular endothelial cell function on materials with various surface roughness. J Biomed Mater Res A 71:154–161
Yang F, Murugan R, Wang S, Ramakrishna S (2005) Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials 26:2603–2610
Yang F, Wolke JGC, Jansen JA (2008) Biomimetic calcium phosphate coating on electrospun poly(ɛ-caprolactone) scaffolds for bone tissue engineering. Chem Eng J 137:154–161
Yang X, Yang F, Walboomers XF, Bian Z, Fan M, Jansen JA (2010) The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. J Biomed Mater Res A 93:247–257
Yao SL, Wang XM, Liu X, Wang RH, Deng CS, Cui FZ (2013) Effects of ambient relative humidity and solvent properties on the. Electrospinning of pure hyaluronic acid nanofibers. J Nanosci Nanotechnol 13:4752–4758
Yeo I-S, Oh J-E, Jeong L, Lee TS, Lee SJ, Park WH et al (2008) Collagen-based biomimetic nanofibrous scaffolds: preparation and characterization of collagen/silk fibroin bicomponent nanofibrous structures. Biomacromolecules 9:1106–1116
Yin A, Zhang K, McClure MJ, Huang C, Wu J, Fang J et al (2013) Electrospinning collagen/chitosan/poly(L-lactic acid-co-epsilon-caprolactone) to form a vascular graft: mechanical and biological characterization. J Biomed Mater Res A 101:1292–1301
Yokoyama Y, Hattori S, Yoshikawa C, Yasuda Y, Koyama H, Takato T et al (2009) Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric. Mater Lett 63:754–756
Yoon H, Kim G (2011) A three-dimensional polycaprolactone scaffold combined with a drug delivery system consisting of electrospun nanofibers. J Pharm Sci 100:424–430
Yoshimoto H, Shin YM, Terai H, Vacanti JP (2003) A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials 24:2077–2082
Yunos DM, Ahmad Z, Salih V, Boccaccini AR (2013) Stratified scaffolds for osteochondral tissue engineering applications: electrospun PDLLA nanofibre coated bioglass(R)-derived foams. J Biomater Appl 27:537–551
Zhang J-G, Mo X-M (2013) Current research on electrospinning of silk fibroin and its blends with natural and synthetic biodegradable polymers. Front Mater Sci 7:129–142
Zhang YJ, Huang YD, Li FF, Wang L, Jin ZH, ieee (2004) Electrospun non-woven mats of EVOH. Isdeiv: xxith international symposium on discharges and electrical insulation in vacuum, vols 1 and 2. Proceedings, Piscataway, New Jersey, pp 106–109
Zhang YZ, Venugopal J, Huang ZM, Lim CT, Ramakrishna S (2006) Crosslinking of the electrospun gelatin nanofibers. Polymer 47:2911–2917
Zhang Y, Venugopal JR, El-Turki A, Ramakrishna S, Su B, Lim CT (2008) Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 29:4314–4322
Zhang F, Zuo B, Fan Z, Xie Z, Lu Q, Zhang X et al (2012a) Mechanisms and control of silk-based electrospinning. Biomacromolecules 13:798–804
Zhang X, Cai Q, Liu H, Zhang S, Wei Y, Yang X et al (2012b) Calcium ion release and osteoblastic behavior of gelatin/beta-tricalcium phosphate composite nanofibers fabricated by electrospinning. Mater Lett 73:172–175
Zhang S, Chen L, Jiang Y, Cai Y, Xu G, Tong T et al (2013) Bi-layer collagen/microporous electrospun nanofiber scaffold improves the osteochondral regeneration. Acta Biomater 9:7236–7247
Zhao L, He C, Gao Y, Cen L, Cui L, Cao Y (2008) Preparation and cytocompatibility of PLGA scaffolds with controllable fiber morphology and diameter using electrospinning method. J Biomed Mater Res B Appl Biomater 87B:26–34
Zhong SP, Teo WE, Zhu X, Beuerman R, Ramakrishna S, Yung LYL (2005) Formation of collagen-glycosaminoglycan blended nanofibrous scaffolds and their biological properties. Biomacromolecules 6:2998–3004
Zhong S, Teo WE, Zhu X, Beuerman RW, Ramakrishna S, Yung LY (2006) An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. J Biomed Mater Res A 79:456–463
Zhou Y, Yang D, Chen X, Xu Q, Lu F, Nie J (2008) Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration. Biomacromolecules 9:349–354
Zhu X, Cui W, Li X, Jin Y (2008) Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules 9:1795–1801
Zong XH, Bien H, Chung CY, Yin LH, Fang DF, Hsiao BS et al (2005) Electrospun fine-textured scaffolds for heart tissue constructs. Biomaterials 26:5330–5338
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Araujo, J.V., Carvalho, P.P., Best, S.M. (2015). Electrospinning of Bioinspired Polymer Scaffolds. In: Bertassoni, L., Coelho, P. (eds) Engineering Mineralized and Load Bearing Tissues. Advances in Experimental Medicine and Biology, vol 881. Springer, Cham. https://doi.org/10.1007/978-3-319-22345-2_3
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