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Performance in the Laboratory

  • Joseph G. SinkovicsEmail author
Chapter

Abstract

Obtaining cellular RNA and DNA. Transfer of genomic DNA from human cancer or leukemic cells into NIH 3T3 cells (established from mouse embryo fibroblasts) resulted in the transformation of 3T3 cells into focus-forming cells. Such focus-forming 3T3 cells first yielded the ras family (Harvey-, Kirsten-, N-ras) oncogenes. Co-transfection of such inoculated 3T3 cells into nude mice increased the sensitivity of the assay.

Keywords

Prostate Cancer Cell Stem Cell Niche Saline Sodium Citrate Mouse Embryo Fibroblast Penicillium Funiculosum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.M.D. Anderson Hospital Comprehensive Cancer CenterThe University of TexasHoustonUSA
  2. 2.H.L. Moffitt Comprehensive Cancer CenterThe University of South FloridaTampaUSA
  3. 3.Department of Molecular MedicineThe University of South Florida Morsani College of MedicineTampaUSA
  4. 4.The Cancer InstituteSt. Joseph’s HospitalTampaUSA

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