Performance in the Laboratory

  • Joseph G. SinkovicsEmail author


Obtaining cellular RNA and DNA. Transfer of genomic DNA from human cancer or leukemic cells into NIH 3T3 cells (established from mouse embryo fibroblasts) resulted in the transformation of 3T3 cells into focus-forming cells. Such focus-forming 3T3 cells first yielded the ras family (Harvey-, Kirsten-, N-ras) oncogenes. Co-transfection of such inoculated 3T3 cells into nude mice increased the sensitivity of the assay.


Prostate Cancer Cell Stem Cell Niche Saline Sodium Citrate Mouse Embryo Fibroblast Penicillium Funiculosum 
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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.M.D. Anderson Hospital Comprehensive Cancer CenterThe University of TexasHoustonUSA
  2. 2.H.L. Moffitt Comprehensive Cancer CenterThe University of South FloridaTampaUSA
  3. 3.Department of Molecular MedicineThe University of South Florida Morsani College of MedicineTampaUSA
  4. 4.The Cancer InstituteSt. Joseph’s HospitalTampaUSA

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