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Coding of Group Odor in the Subcaudal Gland Secretion of the European Badger Meles meles: Chemical Composition and Pouch Microbiota

  • Christina D. BueschingEmail author
  • H. Veronica Tinnesand
  • YungWa Sin
  • Frank Rosell
  • Terry Burke
  • David W. Macdonald

Abstract

The fermentation hypothesis predicts that odor profiles of mammals depend partly on the primary gland products excreted by the animal and partly on the composition of the bacterial flora converting these into secondary metabolites. Some mammalian odors, such as shared group odors, however, need to be consistent yet flexible (e.g., to allow for changes in social-group affiliation), and are thus predisposed for microbial mediation. Using terminal restriction fragment (TRF) length polymorphism analyses we analyzed the microbial community in subcaudal-gland secretions of European badgers (Meles meles) in relation to the chemical scent profiles as determined by gas chromatography-mass spectrometry analyses (GCMS) of 66 adults belonging to six different social groups. We found a total of 50 TRFs and 125 different chemical compounds with a subset of four TRFs best explaining the structure in the chemical matrix. Nevertheless, although semiochemical profiles were group specific, microbial profiles were not. In our approach, however, the number of operational taxonomic units exceeded the numbers of TRFs, and thus our analyses were likely limited by the afforded resolution. As it is likely that the variation in metabolic activity is found at the species-, subspecies-, or even strain-level, future high-throughput sequencing can be expected to reveal more subtle differences in the microbial communities between social groups.

Keywords

Terminal Restriction Fragment Length Polymorphism Scent Mark Scent Profile Terminal Restriction Fragment Length Polymorphism Profile Group Odor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Christina D. Buesching
    • 1
    Email author
  • H. Veronica Tinnesand
    • 2
  • YungWa Sin
    • 1
    • 3
  • Frank Rosell
    • 2
  • Terry Burke
    • 3
  • David W. Macdonald
    • 1
  1. 1.Recanati-Kaplan Centre, Wildlife Conservation Research Unit, Department of ZoologyUniversity of OxfordTubneyUK
  2. 2.Faculty of Arts and Sciences, Department of Environmental and Health StudiesTelemark University CollegeBø, TelemarkNorway
  3. 3.NERC Biomolecular Analysis Facility – Sheffield, Department of Animal & Plant SciencesUniversity of SheffieldSheffieldUK

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