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Basic Principles

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Introduction to Fluorescence Sensing

Abstract

Booming of sensor technologies is a response to a strong demand in society. As a result, almost every physical principle and technique that can detect interactions between molecules, particles and interfaces was suggested and tested for application in sensing. In this Chapter we provide a short survey of basic strategies in molecular sensing and try to determine the role of those of them that are based on fluorescence detection. Some of them are of rather general nature and others are specific for fluorescence technique. They include (a) application of labeled targets in fluorescence assays, (b) competitor displacement assays, (c) sandwich assays, (d) catalytic biosensing and (e) direct reagent-independent sensing. Finally, the reader will find the section “Sensing and thinking” with the list of questions and problems addressed to the readers.

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Authors and Affiliations

  1. Laboratory of Nanobiotechnology Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Alexander P. Demchenko

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  1. Alexander P. Demchenko
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Demchenko, A.P. (2015). Basic Principles. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_1

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