Relationship Between Changes in Contents of Nitric Oxide and Amino Acids Particularly Proline in Plants Under Abiotic Stress

  • David W. M. LeungEmail author


Studies on the physiological response of plants to abiotic stress have identified an array of changes including nitric oxide (NO) generation, accumulation of free proline, reactive oxygen species, antioxidants and oxidative damages. Little is known about the relationships between two of the concurrent changes, NO and proline metabolism. Here, the insights obtained so far and the important research gaps about this were explored.


Crop productivity Morphological alterations l-proline Sodium nitroprusside Stress tolerance 


  1. Ahmad P, Sarwat M, Bhat NA, Wani MR, Kazi AG, Tran LSP (2015) Alleviation of cadmium toxicity in Brassica juncea L. (Czern. & Coss.) by calcium application involves various physiological and biochemical strategies. PLoS One 10:e0114571PubMedCentralCrossRefPubMedGoogle Scholar
  2. Arasimowicz-Jelonek M, Floryszak J, Kubis J (2009) Involvement of nitric oxide in water stress-induced responses in cucumber roots. Plant Sci 177:682–690CrossRefGoogle Scholar
  3. Boldizsar A, Simon-Sarkadi L, Szirtes K, Soltesz A, Szalai G, Keyster M et al (2013) Nitric oxide affects salt-induced changes in free amino acid levels in maize. J Plant Physiol 170:1020–1027CrossRefPubMedGoogle Scholar
  4. Cantrel C, Vazquez T, Puyauberr J, Reze N, Lesch M, Kaiser WM, Dutilleul C, Guillas I, Zachowski A, Baudouin E (2011) Nitric oxide participates in cold-responsive phosphosphingolipid formation and gene expression in Arabidopsis thaliana. New Phytol 189:415–427CrossRefPubMedGoogle Scholar
  5. Deinlein U, Stephan AB, Horie T, Luo W, Xu G, Schroeder JL (2014) Plant salt-tolerance mechanisms. Trends Plant Sci 19:371–379PubMedCentralCrossRefPubMedGoogle Scholar
  6. Du ST, Liu Y, Zhang P, Liu HJ, Zhang XQ, Zhang RR (2015) Atmospheric application of trace amounts of nitric oxide enhances tolerance to salt stress and improves nutritional quality in Spinach (Spinacia oleracea L.). Food Chem 173:905–911CrossRefPubMedGoogle Scholar
  7. Fan HF, Du CX, Guo SR (2012) Effect of nitric oxide on proline metabolism in cucumber seedlings under salinity stress. J Am Soc Hort Sci 137:127–133Google Scholar
  8. Farooq M, Hussain M, Wahid A, Siddique KHM (2012) Drought stress in plants: an overview. In: Aroca R (ed) Plant responses, From morphological to molecular features. Springer, New York, NYGoogle Scholar
  9. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930CrossRefPubMedGoogle Scholar
  10. Gould KS, Lamotte O, Klinguer A, Pugin A, Wendehenne D (2003) Nitric oxide production in tobacco leaf cells: a generalized stress response? Plant Cell Environ 26:1851–1862CrossRefGoogle Scholar
  11. Hatzig S, Zaharia I, Abrams S, Hohmann M, Legoahec L, Bouchereau A, Nesi N, Snowdon RJ (2014) Early osmotic adjustment responses in drought-resistant and drought-sensitive oilseed rape. J Integr Plant Biol 56:797–809CrossRefPubMedGoogle Scholar
  12. Hasanuzzamann M, Nahar K, Alam MM, Fujita M (2014) Modulation of antioxidant machinery and the methylglyoxal detoxification system in selenium-supplemented Brassica napus seedlings confers to high temperature stress. Biol Trace Elem Res 161(Special Issue):297–307CrossRefGoogle Scholar
  13. He JY, Ren YF, Chen XL, Chen H (2014) Protective roles of nitric oxide on seed germination and seedling growth of rice (Oryza sativa L.) under cadmium stress. Ecotoxicol Environ Saf 108:114–119CrossRefPubMedGoogle Scholar
  14. Hu Y, You J, Liang X (2015) Nitrate reductase-mediated nitric oxide production is involved in copper tolerance in shoots of hulless barley. Plant Cell Rep 34:367–379CrossRefPubMedGoogle Scholar
  15. Huang X, Chen MH, Yang LT, Li YR, Wu JM (2015) Effects of exogenous abscisic acid on cell membrane and endogenous hormone contents in leaves of sugarcane seedlings under cold stress. Sugar Tech 17:59–64CrossRefGoogle Scholar
  16. Iqbal N, Umar S, Khan NA, Khan MIR (2014) A new perspective of phytohormones in salinity tolerance: regulation of proline metabolism. Environ Exp Bot 100:34–42CrossRefGoogle Scholar
  17. Kaul S, Sharma SS, Mehta IK (2008) Free radical scavenging potential of l-proline: evidence from in vitro assays. Amino Acids 34:315–320CrossRefPubMedGoogle Scholar
  18. Leung DWM (2015) Regulatory role of nitric oxide (NO) in alterations of morphological features of plants under abiotic stress. In: Khan MN, Mobin M, Mohammad F, Corpas FJ (eds) Nitric oxide action in abiotic stress responses in plants. Springer, New YorkGoogle Scholar
  19. Li X, Gong B, Xu K (2014) Interaction of nitric oxide and polyamines involves antioxidants and physiological strategies against chilling-induced oxidative damage in Zingiber officinale Roscoe. Scientia Hort 170:237–248CrossRefGoogle Scholar
  20. Liu B, Rennenberg H, Kreuzwieser J (2015a) Hypoxia induces stem and leaf nitric oxide (NO) emission from poplar seedlings. Planta 241:579–589CrossRefPubMedGoogle Scholar
  21. Liu SC, Yao MZ, Ma CL, Jin JQ, Ma JQ, Li CF, Chen L (2015b) Physiological changes and differential gene expression of tea plant under dehydration and rehydration conditions. Scientia Hort 184:129–141CrossRefGoogle Scholar
  22. Mahmood T, Gupta KJ, Kaiser WM (2009) Cadmium stress stimulates nitric oxide production. Pak J Bot 41:1285–1290Google Scholar
  23. Munns R, James RA, Lauchli A (2006) Approaches to increasing the salt tolerance in wheat and other cereals. J Exp Bot 57:1025–1043CrossRefPubMedGoogle Scholar
  24. Planchet E, Verdu I, Delahaie J, Cukier C, Girard C, Morere-Le Paven MC, Limami AM (2014) Abscisic acid-induced nitric oxide and proline accumulation in independent pathways under water-deficit stress during seedling establishment in Medicago truncatula. J Exp Bot 65:2161–2170CrossRefPubMedGoogle Scholar
  25. Potters G, Pasternak TP, Guisez Y, Jansen MAK (2009) Different stresses, similar morphogenic responses: integrating a plethora of pathways. Plant Cell Environ 32:158–169CrossRefPubMedGoogle Scholar
  26. Puyaubert J, Baudouin E (2014) New clues for a cold case: nitric oxide response to low temperature. Plant Cell Environ 37:2623–2630CrossRefPubMedGoogle Scholar
  27. Qiao X, Zheng Z, Zhang L, Wang J, Shi G, Xu X (2015) Lead tolerance mechanism in sterilized seedlings of Potamogeton crispus L.: subcellular distribution, polyamines and proline. Chemosphere 120:179–187CrossRefPubMedGoogle Scholar
  28. Rejeb KB, Abdelly C, Savoure A (2014) How reactive oxygen species and proline face stress together. Plant Physiol Biochem 80:278–284CrossRefPubMedGoogle Scholar
  29. Shin JH, Vaughn JN, Abdel-Haleem H, Chavarro C, Abernathy B, Do Kim K, Jackson SA, Li Z (2015) Transcriptome changes due to water deficit define a general soybean response and accession-specific pathways for drought avoidance. BMC Plant Biol 15:26PubMedCentralCrossRefPubMedGoogle Scholar
  30. Sos-Hegedus A, Juhasz Z, Poor P, Kondrak M, Antal F, Tari I (2014) Soil drench treatment with beta-aminobutyric acid increases drought tolerance of potato. PLoS One 9:e114297PubMedCentralCrossRefPubMedGoogle Scholar
  31. Signorelli S, Arellano JB, Melo TB, Borsani O, Monza J (2013a) Proline does not quench singlet oxygen: evidence to reconsider its protective role in plants. Plant Physiol Biochem 64:80–83CrossRefPubMedGoogle Scholar
  32. Signorelli S, Corpas FJ, Borsani O, Barroso JB, Monza J (2013b) Water stress induces a differential and spatially distributed nitro-oxidative stress response in roots and leaves of Lotus japonicus. Plant Sci 201–202:137–146CrossRefPubMedGoogle Scholar
  33. Signorelli S, Coitino EL, Borsani O, Monza J (2014) Molecular mechanisms for the reaction between OH radicals and proline: insights on the role as reactive oxygen species scavenger in plant stress. J Phys Chem 118:37–47CrossRefGoogle Scholar
  34. Talbi S, Romero-Pueras MC, Hernandez A, Terron L, Ferchichi A, Sandalio LM (2015) Drought tolerance in a Saharian plant Oudneya africana: role of antioxidant defences. Environ Exp Bot 111:114–126CrossRefGoogle Scholar
  35. Yang F, Ding F, Duan XH, Zhang J, Li XN, Yang YL (2014) ROS generation and proline metabolism in calli of halophyte Nitraria tangutorum Bobr. to sodium nitroprusside treatment. Protoplasma 251:71–80CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.School of Biological SciencesUniversity of CanterburyChristchurchNew Zealand

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