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Towards Understanding Protein Disorder In-Cell

Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 870)

Abstract

Investigating the activity and structure of cellular biochemical machinery at atomic resolution has been a point of paramount significance for understanding health and disease over the decades. The underlying molecular mechanisms are primarily studied in vitro. Nuclear magnetic resonance (NMR) is a technique that allows to look into cells and study proteins and other constituents, thanks to careful experimental design and technological advances (spectrometer sensitivity and pulse sequence design). Here we outline current applications of the technique and propose a realistic future for the field.

Keywords

In-cell NMR Isotopic labeling Cell types Cell extracts 

Notes

Acknowledgements

Cesyen Cedeño and Hadas Raveh-Amit were fellows in the IDPbyNMR Marie Curie project of the European Commission, 7th Framework Programme (contract no. 264257), and this work has been partially supported by this project. Peter Tompa acknowledges the Research Foundation Flanders (FWO) Odysseus grant G.0029.12.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.VIB Department of Structural BiologyVrije Universiteit BrusselBrusselsBelgium
  2. 2.BioTalentum LtdGodolloHungary
  3. 3.Institute of Enzymology, Biological Research CenterHungarian Academy of SciencesBudapestHungary

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