Abstract
LR White resin is a versatile embedding medium suitable for use in both light and electron microscopy. This embedding medium has a low viscosity, can readily penetrate into dense tissues, and can be polymerized at low and high temperatures using appropriate chemical accelerators and catalysts. The hydrophilic nature of the polymerized resin allows on-section immunostaining and is best suited for the immunogold method of antigen detection. The major characteristics of this embedding medium are discussed and sample protocols for light and electron microscopy are provided in this chapter.
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References
Newman GR, Jasani B, Williams ED (1983) A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochem J 15:543–555
Newman GR, Hobot JA (1987) Modern acrylics for post-embedding immunostaining techniques. J Histochem Cytochem 33:971–981
Newman GR (1987) Use and abuse of LR White. Histochem J 19:118–120
Newman GR, Hobot JA (1993) Resin microscopy and on-section immunocytochemistry. Springer-Verlag, Berlin
Newman GR, Hobot JA (1999) Resins for combined light and electron microscopy: a half century of development. Histochem J 31:495–505
Bell K, Mitchell S, Paultre D, Posch M, Oparka K (2013) Correlative imaging of fluorescent proteins in resin-embedded plant material. Plant Physiol 161:1595–1603
Kuo J (ed) (2014) Electron microscopy, methods and protocols, 3rd edn. Humana Press, New York
Lee KJD, Knox JP (2014) Resin embedding, sectioning, and immunocytichemical analyses of plant cell walls in hard tissues. Meth Mol Biol 1080:41–52
Webster P (2014) Microwave-assisted processing and embedding for transmission electron microscopy. Meth Mol Biol 1117:21–37
Giannoutsou F, Sotiriou P, Apostolakos P, Galatis B (2013) Early local differentiation of the cell wall matrix defines the contact sites in lobed mesophyll cells of Zea mays. Ann Bot 112:1067–1801
Vaughan K (2013) Immunocytochemistry of plant cells. Springer, New York
Wilson SM, Bacic A (2012) Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling pf carbohydrate and protein epitopes. Nat Protoc 7:1716–1727
Marttila S, Santén K (2007) Practical aspects of immunomicroscopy on plant material. In: Mendez-Vilas A, Diaz J (eds) Modern research and educational topics in microscopy. Formatex, Badajoz, pp 1015–1021
Pettigrew WT, Vaughn KC (1998) Physiological, structural, and immunological characterization of leaf and chloroplast development in cotton. Protoplasma 202:23–37
Hoffman JC, Vaughn KC, Joshi HC (1994) Structural and immunocytochemical characterization of microtubule organizing centers in pteridophyte spermatogenous cells. Protoplasma 179:46–60
Satiat-Jeunemaitre B, Hawes C (1992) Redistribution of a golgi glycoprotein in plant cells treated with brefeldine A. J Cell Sci 103:1153–1166
VandenBosch KA (1991) Immunogold labelling. In: Hall JL, Hawes C (eds) Electron microscopy of plant cells. Academic Press, London, pp 181–218
Mollenhauser HH (1993) Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy. Microscopy Res Tech 26:486–512
Hayat MA (2000) Principles and techniques of electron microscopy: biological applications. Cambridge University Press, Cambridge
Maunsbach AB (2006) Immunoelectron microscopy with Lowicryl resins. In: Celis JE (ed) Cell biology, a laboratory handbook, vol 1, 3rd edn. Elsevier Academic Press, Burlington, pp 289–297
Sakai Y, Hosaka M, Hira Y, Watanabe T (2005) Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin. Arch Histol Cytol 68:337–347
McLean W, Nakane PF (1974) Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Palmieri M, Kiss JZ (2005) A novel technique for flat-embedding cryofixed plant specimens in LR White Resin. Microscopy Res Tech 68:80–84
Hand NM (2002) Plastic embedding media and techniques. In: Bancroft JD, Gamble M (eds) Theory and practice of histological techniques. Churchill Livingston, London, pp 663–677
Gerrits PO, Horobin RW (1996) Glycol methacrylate embedding for light microscopy: basic principles and trouble-shooting. J Histotech 19:297–311
Bowling AJ, Vaughn KC (2008) A simple technique to minimize heat damage to specimens during thermal polymerization of LR White in plastic and gelatin capsules. J Microsc 231:186–189
McDonald KL (2014) Rapid embedding methods into epoxy and LR white resins for morphological and immunological analysis of cryofixed biological specimens. Microsc Microanal 20:152–163
Hillmer S, Joachim S, Robinson DG (1991) Rapid polymerization of LR-White for immunocytochemistry. Histochemistry 95:315–318
Reid N (1975) Ultramicrotomy. American Elsevier Pub, Co., New York
Lackie PM (1996) Immunogold silver staining for light microscopy. Histochem Cell Biol 106:9–17
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Yeung, E., Huang, B. (2015). LR White Acrylic Resin. In: Yeung, E., Stasolla, C., Sumner, M., Huang, B. (eds) Plant Microtechniques and Protocols. Springer, Cham. https://doi.org/10.1007/978-3-319-19944-3_6
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DOI: https://doi.org/10.1007/978-3-319-19944-3_6
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