Epoxy Resins for Light and Transmission Electron Microscopy

  • Michael John Sumner


Compared to other epoxy resins, low viscosity Spurr’s resin infiltrates plant tissues more readily and has over the last 45 years become the resin of choice for embedding plant tissues for transmission electron microscopy. This chapter details the six fundamental steps in preparing plant tissues for correlative anatomical studies involving the use of both light and electron microscopy: fixation, dehydration, infiltration, embedding, sectioning and staining.


Diamond knife Lead citrate Transmission electron microscope Spurr’s epoxy resin Toluidine blue O Ultramicrotome 


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of ManitobaWinnipegCanada

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