UV-Visible Spectrophotometry-Based Metabolomic Analysis of Cedrela Fissilis Velozzo (Meliaceae) Calluses - A Screening Tool for Culture Medium Composition and Cell Metabolic Profiles

  • Fernanda Kokowicz PilattiEmail author
  • Christopher Costa
  • Miguel Rocha
  • Marcelo Maraschin
  • Ana Maria Viana
Conference paper
Part of the Advances in Intelligent Systems and Computing book series (AISC, volume 375)


In plant cell cultures aiming at the production of secondary metabolites of industrial interest, the culture medium composition is a decisive step for obtaining cell growth and high yields of the target compound(s). A rapid and reliable methodology for screening metabolic responses to medium composition is fundamental for the development of this biotechnological field. Following this approach, UV-Vis scanning spectrophotometry of callus extracts and their spectra pre-processing, univariate and multivariate analysis were tested in the present work. The results obtained successfully discriminated the culture media investigated and shed light on what metabolic pathways might be responsible for the differences among the callus cultures’ metabolic profiles.


Metabolic profiling Cedrela fissilis UV-Visible spectrophotometry R language Unsupervised methods 



To CAPES (Coordination for the Improvement of Higher Education Personnel) for financial support, Post-Graduation Program in Cell and Developmental Biology (Federal University of Santa Catarina) and BIOSYSTEMS research group (University of Minho).


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Fernanda Kokowicz Pilatti
    • 1
    Email author
  • Christopher Costa
    • 2
  • Miguel Rocha
    • 2
  • Marcelo Maraschin
    • 1
  • Ana Maria Viana
    • 3
  1. 1.Plant Morphogenesis and Biochemistry LaboratoryFederal University of Santa CatarinaFlorianópolisBrazil
  2. 2.Centre Biological Engineering School of EngineeringUniversity of MinhoBragaPortugal
  3. 3.Botany DepartmentFederal University of Santa CatarinaFlorianópolisBrazil

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