Fat Tissue Analysis in the Management of Patients with Systemic Amyloidosis

  • Johan Bijzet
  • Ingrid I. van Gameren
  • Bouke P. C. HazenbergEmail author
Part of the Current Clinical Pathology book series (CCPATH)


Aspiration of abdominal subcutaneous fat tissue is a safe, inexpensive, reliable, minimally invasive, and easy procedure with high diagnostic yield that can be done early after clinical suspicion of amyloidosis and can be repeated if required anytime during the course of the disease. Material obtained in this way is highly suitable for detection, typing, and quantification of amyloid and can also be used for chemical tissue analysis using proteomic techniques. The procedure has great potential to enable dynamic research of local tissue factors involved both in deposition and removal of amyloid in vivo. In the Appendix of this chapter, we describe the current practice of this procedure in our center.


Abdominal subcutaneous fat tissue Fat tissue aspiration Amyloid detection Amyloid typing Congo red stain Birefringence Sensitivity Specificity Accuracy Positive predictive value (PPV) Negative predictive value (NPV) Immunofluorescence Immunohistochemistry Immunoelectron microscopy Immunochemical analysis Cutoff values Reference range Chemical tissue analysis Amino acid sequence Proteomic techniques Men Women Age Number of major organs Follow-up Treatment Survival Disease severity Amyloid quantification Semiquantitative grading Scoring system Amyloid regression Amyloid progression Composition of amyloid Amyloidogenesis 


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© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Johan Bijzet
    • 1
  • Ingrid I. van Gameren
    • 1
  • Bouke P. C. Hazenberg
    • 1
    Email author
  1. 1.Department of Rheumatology & Clinical Immunology, University Medical Center GroningenUniversity of GroningenGroningenThe Netherlands

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