Abstract
The aim of this study was to observe phytoplankton species and associated bacterial populations in the coastal waters of the United Arab Emirates (UAE). Samples were collected from the Umm al Quwain (UAQ) and the Dibba coastal water area. The samples were analyzed to determine the phytoplankton species and associated bacterial populations. A Fluorescent in situ hybridization (FISH) technique, using six sub-groups and a family-specific oligonucleotide probe, was performed on the ethanol and paraformaldehyde fixed samples. The microscope-based analysis of eight samples revealed the presence of both toxic and non-toxic phytoplankton species. The four dominant harmful dinoflagellate species detected in the samples were Cochlodinium polykrikoides, Dinophysis caudata, Prorocentrum arenarium and Protoperidinium spp. The FISH analysis showed a diverse bacterial community associated with the phytoplankton species. The overall findings of this study showed the presence of 4 non-toxic and 11 toxic phytoplankton species in the UAE coastal water samples. The FISH analysis indicated the presence of a highly diverse bacterial community belonging to five different sub-groups (Alpha, Beta and Gamma sub-classes of proteobacteria, High and Low G + C sub-groups) and one family (Enterobacteriaceae).
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Abè TH (1967) The armoured Dinoflagellata: II. Prorocentridae and Dinophysidae (A). Publ Seto Mar Biol Lab 14:369–389
Al Kandari M, Al Yamani F, Al Rifaie K (2009) Marine phytoplankton atlas of Kuwait’s waters. Kuwait Institute of Scientific Research, Safat 54 pp
Al Qubaisi BS (2006) A study of factors influencing the occurrence of Harmful Algae Bloom (HAB) along the Abu Dhabi Coast, UAE. United Arab Emirates University Abu Dhabi: Environment Agency – Abu Dhabi, UAE, 98 pp
Amann RI, Krumholz L, Stahl DA (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental-studies in microbiology. J Bacteriol 172:762–770
Arnold T (2009) Red tide forces closure of Dubai beaches. Retrieved February 10, 2014, from ArabianBusiness.com: http://www.arabianbusiness.com/551844-red-tide-forces-closure-of-dubai-beach
Azam F, Fenchel T, Field JG, Gray JS, Meyer-Reil LA, Thingstad F (1983) The ecological role of water-column microbes in the sea. Mar Ecol Prog Ser 10:257–263
Barlaan EA, Furukawa S, Takeuchi K (2007) Detection of bacteria associated with harmful algal blooms from coastal and microcosm environments using electronic microarrays. Environ Microbiol 9:690–702
Bauman AG, Burt JA, Feary DA, Marquis E, Usseglio P (2010) Tropical harmful algal blooms: an emerging threat to coral reef communities. Mar Pollut Bull 60:2117–2122
Cho SY, Nagai S, Han MS (2009) Development of microsatellite markers in red-tide causative species Prorocentrum micans (Dinophyceae). Conserv Genet 10:1151–1153
Daims H, Bruhl A, Amann RI, Schleifer KH, Wagner M (1999) The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22:434–444
Daims H, Stoecker K, Wagner M (2005) Fluorescence in situ hybridization for the detection of prokaryotes. In: Osborn AM, Smith CJ (eds) Advanced methods in molecular microbial ecology. Bios-Garland, Abingdon, pp 213–239
Doucette GJ, Kodama M, Gallacher S (1998) Bacterial interaction with harmful algal bloom species: bloom ecology, toxigenesis and cytology. In: Anderson DM, Cembella AD, Hallegraeff GM (eds) Physiological ecology of harmful algal bloom. Springer, Heidelberg/Berlin, pp 619–647
Fuad A (2008) Weather change ‘will end worst red tide in year’. Retrieved February 10, 2014, from Gulfnews.com: http://gulfnews.com/news/gulf/uae/environment/weather-change-will-end-worst-red-tide-in-years-1.143828
Fukuyo Y, Takano H, Chihara M, Matsuoka K (1990) Red tide organisms in Japan. An illustrated taxonomic guide, Uchida Rokakuho, Co., Ltd., Tokyo, 407 pp
Glibert PM (2007) Eutrophication and Harmful Algal Blooms: a complex global issue, examples from the Arabian Seas including Kuwait Bay, and an introduction to the Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) programme. Int J Oceans Oceanogr 2:157–169
Graneli E, Sundstrom B, Edler L, Anderson DM (1990) Toxic marine phytoplankton. Elsevier, New York, 554 pp
Hold GL, Smith EA, Rappé MS, Maas EW, Moore ERB, Stroempl C, Stephen JR, Prosser JI, Birkbeck TH, Gallacher S (2001) Characterisation of bacterial communities associated with toxic and non-toxic dinoflagellates: Alexandrium spp. and Scrippsiella trochoidea. FEMS Microbiol Ecol 37:161–173
Kudela RM, Gobler CJ (2012) Harmful dinoflagellate blooms caused by Cochlodinium sp.: global expansion and ecological strategies facilitating bloom formation. Harmful Algae 14:71–86
Landais E (2008) Dead fish floating in Dubai creek. Retrieved February 10, 2014, from Gulf News: http://www.gulfnews.com/Nation/Environment/10227257.html
Liu J, Lewitus AJ, Kempton JW, Wilde SB (2008) The association of algicidal bacteria and raphidophyte blooms in South Carolina brackish detention ponds. Harmful Algae 7:184–193
Manz W, Amann R, Ludwig W, Wagner M, Schleifer KH (1992) Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst Appl Microbiol 15:593–600
Meier H, Amann RI, Ludwig W, Schleifer KH (1999) Specific oligonucleotide probes for in situ detection of a major group of gram-positive bacteria with low DNA G + C content. Syst Appl Microbiol 22:186–196
Nishitani G, Nagai S, Sakiyama S, Kamiyama T (2008) Successful cultivation of the toxic dinoflagellate Dinophysis caudata (Dinophyceae). Plankton Benthos Res 3:78–85
Okaichi T (1967) Red tides found in and around the Seto Inland Sea in 1965. Tech Bull Fac Agric Kagawa Univ 15:181–185
Rajan A, Al Abdessalaam TZ (2008) Guide to common marine phytoplankton in Abu Dhabi waters. Environmental Agency-Abu Dhabi, 127 pp
Richlen ML, Morton SL, Jamali EA, Rajan A, Anderson DM (2010) The catastrophic 2008–2009 red tide in the Arabian gulf region, with observations on the identification and phylogeny of the fish-killing dinoflagellate Cochlodinium polykrikoides. Harmful Algae 9:163–172
Romalde J, Barja J, Toranzo AE (1990) Vibrios associated with red tides caused by Mesodinium rubrum. Appl Environ Microbiol 56:3615–3619
Schuppler M, Wagner M, Schon G, Gobel UB (1998) In situ identification of nocardioform actinomycetes in activated sludge using fluorescent rRNA-targeted oligonucleotide probes. Microbiology 144:249–259
Su JQ, Yang XR, Zhou Y, Zheng T (2011) Marine bacteria antagonistic to the harmful algal bloom species Alexandrium tamarense (Dinophyceae). Biol Control 56:132–138
Tomas CR (1997) Identifying marine phytoplankton. Academic, New York, 858 pp
Xin W, Yun T (2009) Lysis of a red tide causing algae, caused by bacteria from its phycosphere. Biol Control 52:123–130
Yang C, Zhou Yi Li Y, Zheng W, Tian Y, Zheng T (2012) Bacterial community dynamics during a bloom caused by Akashiwo sanguinea in the Xiamen Sea area, China. Harmful Algae 20:132–141
Yoo A (2009) Red tide rising: an investigation of recent increased Harmful Algal Blooms in the United Arab Emirates. Global climate change: the science, social impact and diplomacy of a world environmental crisis. Graduate Research Paper, Harvard University, August 5, 2009. Retrieved February 10, 2014, from: http://www.climate-talks.net/2013-ENVRE130/PDF/YOO_GRADUATE_PROJECT_S-130.pdf
Zhao J, Ghedira H (2014) Monitoring red tide with satellite imagery and numerical models: a case study in the Arabian Gulf. Mar Pollut Bull 79(1–2):305–313
Acknowledgement
We are thankful to the Ministry of Environment and Water (MOEW), Dubai, UAE for permission to carry out a part of this work at the Marine resources research centre, Umm Al Quwain, UAE. We are grateful to Dr. Rashid Mohammed AlShihi and Dr. Jaishinimol Santhamma (Ministry of Environment and Water, UAE) for their assistance during the collection and analysis of samples in the Marine resources research centre laboratory, Umm Al Quwain, UAE. We appreciate Dr. Robert Boldi’s valuable comments for the improvement of the manuscript.
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Fig. 17.1 Dominant harmful marine dinoflagellates species found in the samples (a) Cochlodinium polykrikoides (b) Dinophysis caudata (c) Prorocentrum arenarium (d) Protoperidinium spp.
Fig. 17.2 Whole cell rRNA targeted fluorescence in situ hybridization of bacterial community members belonging to: (a), Alpha sub-class of proteobacteria (b) Beta sub-class of proteobacteria (c) Gamma sub-class of proteobacteria (d) Enterobacteriaceae family (e) High G + C sub-class (f) Low G + C sub-class. All samples were hybridized by the sub-class specific TRITC–labelled oligonucleotide probe. For each panel, identical field was viewed by epifluorescence microscopy. Bar = 10 μm applies to all photomicrographs. Original magnification: 1000×
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Khan, M.A., Qalandri, K.G.A., Sankaran, A., Adnani, L.H., AlAlami, U. (2015). Phytoplankton Species and Associated Bacterial Populations in the Coastal Water of the United Arab Emirates. In: Baawain, M., Choudri, B., Ahmed, M., Purnama, A. (eds) Recent Progress in Desalination, Environmental and Marine Outfall Systems. Springer, Cham. https://doi.org/10.1007/978-3-319-19123-2_17
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