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Intranasal Osteopontin for Rodent Germinal Matrix Hemorrhage

Chapter
Part of the Acta Neurochirurgica Supplement book series (NEUROCHIRURGICA, volume 121)

Abstract

Germinal matrix hemorrhage (GMH) is the most common and devastating neurological problem of premature infants. Current treatment is largely ineffective and GMH has been nonpreventable. Osteopontin (OPN) is an endogenous protein that has been shown to be neuroprotective, however, it has not been tested in GMH. P7 neonatal rats were subjected to stereotactic ganglionic eminence collagenase infusion. Groups were as follows: (1) sham, (2) GMH + vehicle, (3) GMH + intranasal OPN. Seventy-two hours later, the animals were evaluated using righting reflex, blood-brain barrier (BBB) permeability by Evans blue dye leakage, brain water content, and hemoglobin assay. Intranasal OPN improved outcomes after GMH by attenuation of brain swelling, BBB function, re-bleeding, and neurological outcomes. OPN may play an important role in enhancing neuroprotective brain signaling following GMH. These observed effects may offer novel possibilities for therapy in this patient population.

Keywords

Stroke, experimental Osteopontin Neonatal rats Germinal matrix hemorrhage Hydrocephalus Neurological dysfunction 
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Notes

Acknowledgment

This study was partially supported by the National Institutes of Health grant RO1 NS078755 (Dr. Zhang) and American Heart Association CRP 17380009 (Dr. Lekic).

Disclosures

None

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Division of Physiology and PharmacologySchool of MedicineLoma LindaUSA
  2. 2.Departments of NeonatologySchool of MedicineLoma LindaUSA
  3. 3.NeurosurgerySchool of MedicineLoma LindaUSA
  4. 4.Department of PhysiologyLoma Linda University School of MedicineLoma LindaUSA

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