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Table 10.1 Properties of Caco-2 cells

From: Caco-2 Cell Line

Growth Grows in culture as an adherent monolayer of epithelial cells
Differentiation Takes 14–21 days after confluence under standard culture conditions
Cell morphology Polarized cells with tight junctions and brush border at the apical side
Electrical parameters High electrical resistance
Digestive enzymes Expresses typical digestive enzymes, membrane peptidases and disaccharidases of the small intestine (lactase, aminopeptidase N, sucrase-isomaltase and dipeptidylpeptidase IV)
Active transport Amino acids, sugars, vitamins, hormones
Membrane ionic transport Na+/K+ ATPase, H+/K+ ATPase, Na+/H+ exchange, Na+/K+/Cl cotransport, apical Cl channels
Membrane non-ionic transporters Permeability glycoprotein (P-gp, multidrug resistance protein), multidrug resistance-associated protein, lung cancer-associated resistance protein
Receptors Vitamin B12, vitamin D3, EGFR (epidermal growth factor receptor), sugar transporters (GLUT1, GLUT2, GLUT3, GLUT5, SGLT1)
Cytokine production IL-6, IL-8, TNFα, TGF-β1, thymic stromal lymphopoietin (TSLP), IL-15