Skip to main content
SpringerLink
Log in

Translation of Therapeutic Antibodies for Intraoperative Fluorescence Imaging

  • Chapter
Fluorescence Imaging for Surgeons

  • 1184 Accesses

Abstract

Preclinical data in fluorescence-guided surgery have provided strong evidence that real-time navigation for cancer localization is highly feasible. However, many of these cancer-specific probes are likely to be expensive and time-consuming to translate to the clinic. To facilitate the translation of cancer-specific localization of fluorescence for surgical navigation, therapeutic antibodies are being coupled with fluorescent molecules to provide robust contrast between disease and normal tissue. In this chapter, specific components of the antibody-based approach are detailed to provide an overview of the requirements for successful translation into human use.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Zinn KR, Korb M, Samuel S, Warram JM, Dion D, Killingsworth C, et al. IND-directed safety and biodistribution study of intravenously injected Cetuximab-IRDye800 in Cynomolgus Macaques. Mol Imaging Biol. 2014;17:49–57.

    Article  Google Scholar 

  2. Cohen R, Stammes MA, de Roos IH, Stigter-van Walsum M, Visser GW, van Dongen GA. Inert coupling of IRDye800CW to monoclonal antibodies for clinical optical imaging of tumor targets. EJNMMI Res. 2011;1(1):31.

    Article  PubMed Central  PubMed  Google Scholar 

  3. Day KE, Beck LN, Heath CH, Huang CC, Zinn KR, Rosenthal EL. Identification of the optimal therapeutic antibody for fluorescent imaging of cutaneous squamous cell carcinoma. Cancer Biol Ther. 2013;14(3):271–7.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Heath CH, Deep NL, Beck LN, Day KE, Sweeny L, Zinn KR, et al. Use of panitumumab-IRDye800 to image cutaneous head and neck cancer in mice. Otolaryngol Head Neck Surg. 2013;148(6):982–90.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Heath CH, Deep NL, Sweeny L, Zinn KR, Rosenthal EL. Use of panitumumab-IRDye800 to image microscopic head and neck cancer in an orthotopic surgical model. Ann Surg Oncol. 2012;19(12):3879–87.

    Article  PubMed Central  PubMed  Google Scholar 

  6. Korb ML, Hartman YE, Kovar J, Zinn KR, Bland KI, Rosenthal EL. Use of monoclonal antibody-IRDye800CW bioconjugates in the resection of breast cancer. J Surg Res. 2014;188(1):119–28.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Messali A, Villacorta R, Hay JW. A review of the economic burden of glioblastoma and the cost effectiveness of pharmacologic treatments. Pharmacoeconomics. 2014;32:1201–12.

    Article  PubMed  Google Scholar 

  8. Liu JT, Meza D, Sanai N. Trends in fluorescence image-guided surgery for gliomas. Neurosurgery. 2014;75(1):61–71.

    Article  PubMed Central  PubMed  Google Scholar 

  9. McClain SE, Mayo KB, Shada AL, Smolkin ME, Patterson JW, Slingluff Jr CL. Amelanotic melanomas presenting as red skin lesions: a diagnostic challenge with potentially lethal consequences. Int J Dermatol. 2012;51(4):420–6.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Rosenthal EL, Kulbersh BD, Duncan RD, Zhang W, Magnuson JS, Carroll WR, et al. In vivo detection of head and neck cancer orthotopic xenografts by immunofluorescence. Laryngoscope. 2006;116(9):1636–41.

    Article  PubMed  Google Scholar 

  11. Rosenthal EL, Kulbersh BD, King T, Chaudhuri TR, Zinn KR. Use of fluorescent labeled anti-epidermal growth factor receptor antibody to image head and neck squamous cell carcinoma xenografts. Mol Cancer Ther. 2007;6(4):1230–8.

    Article  CAS  PubMed  Google Scholar 

  12. Stummer W, Pichlmeier U, Meinel T, Wiestler OD, Zanella F, Reulen HJ, et al. Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial. Lancet Oncol. 2006;7(5):392–401.

    Article  CAS  PubMed  Google Scholar 

  13. van Dam GM, Themelis G, Crane LM, Harlaar NJ, Pleijhuis RG, Kelder W, et al. Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-alpha targeting: first in-human results. Nat Med. 2011;17(10):1315–9.

    Article  PubMed  Google Scholar 

  14. Bailey KM, Wojtkowiak JW, Hashim AI, Gillies RJ. Targeting the metabolic microenvironment of tumors. Adv Pharmacol. 2012;65:63–107.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Zhan X, Lin Y, Gillies RJ. Tumor pH and its measurement. J Nucl Med. 2010;51(8):1167–70.

    Article  Google Scholar 

  16. Gindy ME, Prud’homme RK. Multifunctional nanoparticles for imaging, delivery and targeting in cancer therapy. Expert Opin Drug Deliv. 2009;6(8):865–78.

    Article  CAS  PubMed  Google Scholar 

  17. Jiang S, Gnanasammandhan MK, Zhang Y. Optical imaging-guided cancer therapy with fluorescent nanoparticles. J R Soc Interface. 2010;7(42):3–18.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Adams KE, Ke S, Kwon S, Liang F, Fan Z, Lu Y, et al. Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer. J Biomed Opt. 2007;12(2):024017.

    Article  PubMed  Google Scholar 

  19. Kramer-Marek G, Longmire MR, Choyke PL, Kobayashi H. Recent advances in optical cancer imaging of EGF receptors. Curr Med Chem. 2012;19(28):4759–66.

    Article  CAS  PubMed  Google Scholar 

  20. Andrades P, Bohannon IA, Baranano CF, Wax MK, Rosenthal E. Indications and outcomes of double free flaps in head and neck reconstruction. Microsurgery. 2009;29(3):171–7.

    Article  PubMed  Google Scholar 

  21. Newman JR, Gleysteen JP, Barañano CF, Bremser JR, Zhang W, Zinn KR, et al. Stereomicroscopic fluorescence imaging of head and neck cancer xenografts targeting CD147. Cancer Biol Ther. 2008;7(7):1063–70.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Shah N, Zhai G, Knowles JA, Stockard CR, Grizzle WE, Fineberg N, et al. (18)F-FDG PET/CT imaging detects therapy efficacy of anti-EMMPRIN antibody and gemcitabine in orthotopic pancreatic tumor xenografts. Mol Imaging Biol. 2012;14(2):237–44.

    Article  PubMed Central  PubMed  Google Scholar 

  23. Withrow KP, Newman JR, Skipper JB, Gleysteen JP, Magnuson JS, Zinn K, et al. Assessment of bevacizumab conjugated to Cy5.5 for detection of head and neck cancer xenografts. Technol Cancer Res Treat. 2008;7(1):61–6.

    Article  CAS  PubMed  Google Scholar 

  24. Weissleder R. Molecular imaging in cancer. Science. 2006;312(5777):1168–71.

    Article  CAS  PubMed  Google Scholar 

  25. Agdeppa ED, Spilker ME. A review of imaging agent development. AAPS J. 2009;11(2):286–99.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Sliwkowski MX, Mellman I. Antibody therapeutics in cancer. Science. 2013;341(6151):1192–8.

    Article  CAS  PubMed  Google Scholar 

  27. Helman EE, Newman JR, Dean NR, Zhang W, Zinn KR, Rosenthal EL. Optical imaging predicts tumor response to anti-EGFR therapy. Cancer Biol Ther. 2010;10(2):166–71.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Netti PA, Roberge S, Boucher Y, Baxter LT, Jain RK. Effect of transvascular fluid exchange on pressure-flow relationship in tumors: a proposed mechanism for tumor blood flow heterogeneity. Microvasc Res. 1996;52(1):27–46.

    Article  CAS  PubMed  Google Scholar 

  29. van der Vorst JR, Schaafsma BE, Verbeek FP, Keereweer S, Jansen JC, van der Velden LA, et al. Near-infrared fluorescence sentinel lymph node mapping of the oral cavity in head and neck cancer patients. Oral Oncol. 2013;49(1):15–9.

    Article  PubMed Central  PubMed  Google Scholar 

  30. Tanaka E, Choi HS, Humblet V, Ohnishi S, Laurence RG, Frangioni JV. Real-time intraoperative assessment of the extrahepatic bile ducts in rats and pigs using invisible near-infrared fluorescent light. Surgery. 2008;144(1):39–48.

    Article  PubMed Central  PubMed  Google Scholar 

  31. Tanaka E, Ohnishi S, Laurence RG, Choi HS, Humblet V, Frangioni JV. Real-time intraoperative ureteral guidance using invisible near-infrared fluorescence. J Urol. 2007;178(5):2197–202.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Snoeks TJ, van Driel PB, Keereweer S, Aime S, Brindle KM, van Dam GM, et al. Towards a successful clinical implementation of fluorescence-guided surgery. Mol Imaging Biol. 2014;16(2):147–51.

    Article  CAS  PubMed  Google Scholar 

  33. Millesi M, Kiesel B, Woehrer A, Hainfellner JA, Novak K, Martínez-Moreno M, et al. Analysis of 5-aminolevulinic acid-induced fluorescence in 55 different spinal tumors. Neurosurg Focus. 2014;36(2):E11.

    Article  PubMed  Google Scholar 

  34. Stummer W, Novotny A, Stepp H, Goetz C, Bise K, Reulen HJ. Fluorescence-guided resection of glioblastoma multiforme by using 5-aminolevulinic acid-induced porphyrins: a prospective study in 52 consecutive patients. J Neurosurg. 2000;93(6):1003–13.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, The University of Alabama at Birmingham (UAB), BDB 563, 1720 2nd Ave S, Birmingham, AL, 35294-0012, USA

    Eben L. Rosenthal M.D. & Jason M. Warram Ph.D.

  2. Division of Otolaryngology, The University of Alabama at Birmingham (UAB), BDB 563, 1720 2nd Ave S, Birmingham, AL, 35294-0012, USA

    Eben L. Rosenthal M.D. & Jason M. Warram Ph.D.

Authors
  1. Eben L. Rosenthal M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jason M. Warram Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eben L. Rosenthal M.D. .

Editor information

Editors and Affiliations

  1. Section of Minimally Invasive Surgery, Department of General and Vascular Surgery, The Bariatric and Metabolic Institute, Cleveland Clinic Florida, FL, USA; Oncological Surgical Division, Division of Surgical Research, Department of Surgery, Hospital de Clinicas Buenos Aires, University of Buenos Aires, Buenos Aires, Argentina

    Fernando D. Dip

  2. Department of Gastroenterological Surgery, Cancer Institute Hospital, Japanese Foundation for Cancer Research; Hepato-Biliary-Pancreatic Surgery, Department of Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan

    Takeaki Ishizawa

  3. Hepato-Biliary-Pancreatic Surgery; Department of Surgery, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Norihiro Kokudo

  4. Department of General Surgery, Section of Minimally Invasive Surgery; Department of General and Vascular Surgery, The Bariatric and Metabolic Institute, Cleveland Clinic Florida, Weston, Florida, USA

    Raul J. Rosenthal

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Rosenthal, E.L., Warram, J.M. (2015). Translation of Therapeutic Antibodies for Intraoperative Fluorescence Imaging. In: Dip, F., Ishizawa, T., Kokudo, N., Rosenthal, R. (eds) Fluorescence Imaging for Surgeons. Springer, Cham. https://doi.org/10.1007/978-3-319-15678-1_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-15678-1_3

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-15677-4

  • Online ISBN: 978-3-319-15678-1

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation