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Taurine 9 pp 473-480 | Cite as

Effects of Chronic Taurine Administration on Gene Expression, Protein Translation and Phosphorylation in the Rat Hippocampus

  • Atsushi Toyoda
  • Hiroaki Koike
  • Kouichiro Nishihata
  • Wataru Iio
  • Tatsuhiko Goto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 803)

Abstract

Taurine is one of the most abundant amino acids in the brain. It plays various important physiological functions as a neuromodulator and antioxidant. Taurine is suspected to be involved in depression as families that suffer from a hereditary taurine deficiency have a tendency to develop depression. However, knowledge regarding its function in relation to depression is limited. Previously, we reported that the chronic oral administration of taurine has an anti-depressant-like effect in rats and changes the phosphorylation levels of hippocampal signal transduction molecules including ERK and CaMKII. In this study, we studied the global changes of expression in hippocampal proteins and genes under chronic taurine supplementation. Wistar male rats were fed taurine-contained diet for 4 weeks. Hippocampal gene, protein expression and their phosphorylation levels were evaluated by the commercial microarrays. Some prominent changes of protein phosphorylation and expression levels in the taurine-fed rats were observed by the antibody microarray. Especially, hippocampal p53, Jun, PKCs and HDAC5 were overexpressed and/or phosphorylated in the taurine-fed rats compared to control. Also, DNA microarray showed some changes of hippocampal gene expression in the taurine-fed rats. In conclusion, our findings suggest that chronic taurine supplementation has an antidepressant-like effect via some changes of expression and phosphorylation of the hippocampal proteins.

Keywords

Animal model Antibody Depression HDAC5 Microarray Hippocampus p53 Phosphorylation Rat Social defeat 

Abbreviations

Akt

Protein kinase B

BDNF

Brain-derived neurotrophic factor

CaMKII

Calcium/calmodulin-dependent protein kinase II

CCL6

Chemokine (C-C motif) ligand 6

CD80

Cluster of differentiation 80

CMTM2a

Chemokine-like factor-like MAL and related proteins for vesicle trafficking and membrane link domain transmembrane domain containing 2a

CRABP1

Cellular retinoic acid-binding protein 1

CREB

cAMP-response element-binding protein

EAPP

E2F-associated phosphoprotein

ERK1/2

Extracellular signal-regulated kinase1/2

GSK3β

Glycogen synthase kinase3-beta

HDAC

Histone deacetylase

PKC

Protein kinase C

PLAC8

Placentanspecific 8

QOL

Quality of life

SPP1

Secreted phosphoprotein 1

SSRI

Selective serotonin reuptake inhibitor

Notes

Acknowledgements

We would like to thank Drs. Masaya Katsumata (Azabu University, Japan) and Teruo Miyazaki (Tokyo Medical University, Japan) for helpful comments regarding the manuscript. This research was supported in part by Ibaraki University Cooperation between Agriculture and Medicine (IUCAM) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Morinaga Houshikai (Japan).

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Atsushi Toyoda
    • 1
    • 2
    • 3
  • Hiroaki Koike
    • 2
  • Kouichiro Nishihata
    • 2
  • Wataru Iio
    • 1
    • 2
  • Tatsuhiko Goto
    • 2
    • 3
  1. 1.United Graduate School of Agricultural ScienceTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.College of AgricultureIbaraki UniversityInashikiJapan
  3. 3.Ibaraki University Cooperation between Agriculture and Medicine (IUCAM)InashikiJapan

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