Abstract
We present a technique that records transient changes in the concentration of free Ca2+ in live neurons with spatial resolution along a one-dimensional scan. The technique is based on recording fluorescence lifetime changes of a Ca2+ probe by multi-dimensional TCSPC. The sample is scanned with a high-frequency pulsed laser beam, single photons of the fluorescence light are detected, and a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a periodical stimulation of the sample is built up. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about 1 ms.
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Frere, S., Slutsky, I. (2015). Calcium Imaging Using Transient Fluorescence-Lifetime Imaging by Line-Scanning TCSPC. In: Becker, W. (eds) Advanced Time-Correlated Single Photon Counting Applications. Springer Series in Chemical Physics, vol 111. Springer, Cham. https://doi.org/10.1007/978-3-319-14929-5_5
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DOI: https://doi.org/10.1007/978-3-319-14929-5_5
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