Tracking Red Blood Cells Flowing through a Microchannel with a Hyperbolic Contraction: An Automatic Method
The present chapter aims to assess the motion and deformation index of red blood cells (RBCs) flowing through a microchannel with a hyperbolic contraction using an image analysis based method. For this purpose, a microchannel containing a hyperbolic contraction was fabricated in polydimethylsiloxane by using a soft-lithography technique and the images were captured by a standard high-speed microscopy system. An automatic image processing and analyzing method has been developed in a MATLAB environment, not only to track both healthy and exposed RBCs motion but also to measure the deformation index along the microchannel. The keyhole model has proved to be a promising technique to track automatically healthy and exposed RBCs flowing in this kind of microchannels.
KeywordsOptical Flow Bilateral Filter Atomic Region Motion Segmentation Deformation Index
The authors acknowledge the financial support provided by PTDC/SAUBEB/105650/2008, PTDC/SAU-ENB/116929/2010, EXPL/EMS-SIS/2215/2013 from FCT (Science and Technology Foundation), COMPETE, QREN and European Union (FEDER).
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