Abstract
The binding constant and the speed of reaction of proteins and enzymes are pivotal characteristics for some proteins. Such informations are important for example in pharmaceutical research when characterizing the mode of action and the half-life of drugs. Mass spectrometry is a useful tool that could be utilized in the analysis of the binding of compounds of interest to a protein or a receptor. It could also be used to decipher the reaction constants of enzymes. Developmental methods contributed to study and describe the optimal conditions for such analyses. This chapter presents process established to study enzymatic characteristics as well as improvements in terms of mass spectrometry-based study of binding constants.
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Pottiez, G. (2015). How to Determine Protein Function by Mass Spectrometry?. In: Mass Spectrometry: Developmental Approaches to Answer Biological Questions. SpringerBriefs in Bioengineering. Springer, Cham. https://doi.org/10.1007/978-3-319-13087-3_6
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DOI: https://doi.org/10.1007/978-3-319-13087-3_6
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