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Chaga (Inonotus Obliquus) Mushroom: Nutraceutical Assesement Based on Latest Findings

  • Seema PatelEmail author
Chapter
Part of the Applied Environmental Science and Engineering for a Sustainable Future book series (AESE)

Abstract

Chaga mushroom (Inonotus obliquus) is a white-rot fungus growing on birch trees. It looks like burnt coal, has edibility and is regarded a traditional medicinal mushroom in China, Russia, Poland, other Baltic countries. It is hailed as a folk remedy against diabetes, digestive disorders, intestinal worms, liver ailments, cardiovascular diseases, tuberculosis and cancer etc. Recently, it has grabbed attention from mainstream medicine as a therapeutic mushroom. The discovery of a diverse range of secondary metabolites including, phenolic compounds, polysaccharides, melanins, lanosterol, inotodiol, ergosterol and trametenolic acid have promoted it as a potential drug candidate. Biological roles as antioxidant, antiinflammation, anticancer, immunomodulation, antiallergy, antilipemic, hypoglycaemic, antiviral, neurostimulation have been validated. This chapter delves into the biological roles and mechanisms governing the effects.

Keywords

Chaga Inonotus obliquus Polysaccharide Antioxidant anticancer Antiviral 

References

  1. Chen Y, Gu X, Huang SQ, Li J, Wang X, Tang J (2010a) Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities. Int J Biol Macromol 46:429–435CrossRefGoogle Scholar
  2. Chen H, Xu X, Zhu Y (2010b) Optimization of hydroxyl radical scavenging activity of exo-polysaccharides from Inonotus obliquus in submerged fermentation using response surface methodology. J Microbiol Biotechnol 20:835–843Google Scholar
  3. Chen H, Yan M, Zhu J, Xu X (2011) Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of Inonotus obliquus by lignocellulose decomposition. J Ind Microbiol Biotechnol 38:291–298CrossRefGoogle Scholar
  4. Choi SY, Hur SJ, An CS, Jeon YH, Jeoung YJ, Bak JP, Lim BO (2010) Anti-inflammatory effects of Inonotus obliquus in colitis induced by dextran sodium sulfate. J Biomed Biotechnol doi:10.1155/2010/943516Google Scholar
  5. Chung MJ, Chung CK, Jeong Y, Ham SS (2010) Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells. Nutr Res Pract 4:177–182CrossRefGoogle Scholar
  6. Debnath T, Hasnat MA, Pervin M, Lee SY, Park SR, Kim DH, Kweon HJ, Kim JM, Lim BO (2012) Chaga mushroom (Inonotus obliquus) grown on germinated brown rice suppresses inflammation associated with colitis in mice. Food Sci Biotechnol 21:1235–1241CrossRefGoogle Scholar
  7. Du D, Zhu F, Chen X, Ju X, Feng Y, Qi LW, Jiang J (2011) Rapid isolation and purification of inotodiol and trametenolic acid from Inonotus obliquus by high-speed counter-current chromatography with evaporative light scatting detection. Phytochem Anal 22:419–423CrossRefGoogle Scholar
  8. Fan L, Ding S, Ai L, Deng K (2012) Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus. Carbohydr Polym 90:870–874CrossRefGoogle Scholar
  9. Fu L, Chen H, Dong P, Zhang X, Zhang M (2010) Effects of ultrasonic treatment on the physicochemical properties and DPPH radical scavenging activity of polysaccharides from mushroom Inonotus obliquus. J Food Sci 75:322–327CrossRefGoogle Scholar
  10. Gao Y, Xu H, Lu Z, Xu Z (2009) Quantitative determination of steroids in the fruiting bodies and submerged-cultured mycelia of Inonotus obliquus. Se Pu 27:745–749Google Scholar
  11. Geng Y, Lu ZM, Huang W, Xu HY, Shi JS, Xu ZH (2013) Bioassay-guided isolation of DPP-4 inhibitory fractions from extracts of submerged cultured of Inonotus obliquus. Molecules 18:1150–1161CrossRefGoogle Scholar
  12. Giridharan VV, Thandavarayan RA, Konishi T (2011) Amelioration of scopolamine induced cognitive dysfunction and oxidative stress by Inonotus obliquus—a medicinal mushroom. Food Funct 2: 20–327CrossRefGoogle Scholar
  13. Gracheva NV, Golovanchikov AB (2011) Studies of the intensification of the extraction of biologically active substances from chaga using direct current electric fields. Pharm Chem J 44:608–610CrossRefGoogle Scholar
  14. Gunjima K, Tomiyama R, Takakura K, Yamada T, Hashida K, Nakamura Y, Konishi T, Matsugo S, Hori O (2013) 3,4-dihydroxybenzalacetone protects against Parkinson’s disease-related neurotoxin 6-OHDA through Akt/Nrf2/glutathione pathway. J Cell Biochem doi:10.1002/jcb.24643Google Scholar
  15. Ham SS, Kim SH, Moon SY, Chung MJ, Cui CB, Han EK, Chung CK, Choe M (2009) Antimutagenic effects of subfractions of Chaga mushroom (Inonotus obliquus) extract. Mutat Res 672:55–59CrossRefGoogle Scholar
  16. Handa N, Yamada T, Tanaka R (2010) An unusual lanostane-type triterpenoid, spiroinonotsuoxodiol, and other triterpenoids from Inonotus obliquus. Phytochemistry 71:1774–1779CrossRefGoogle Scholar
  17. Huang SQ, Ding S, Fan L (2012) Antioxidant activities of five polysaccharides from Inonotus obliquus. Int J Biol Macromol 50:1183–1187CrossRefGoogle Scholar
  18. Kim JH, Sung NY, Kwon SK, Srinivasan P, Song BS, Choi JI, Yoon Y, Kim JK, Byun MW, Kim MR, Lee JW (2009) Gamma-irradiation improves the color and antioxidant properties of Chaga mushroom (Inonotus obliquus) extract. J Med Food 12:1343–1347CrossRefGoogle Scholar
  19. Kim YJ, Park J, Min BS, Shim SH (2011) Chemical constituents from the sclerotia of Inonotus obliquus. J Korean Soc Appl Biol Chem 54:287–294Google Scholar
  20. Ko SK, Jin M, Pyo MY (2011) Inonotus obliquus extracts suppress antigen-specific IgE production through the modulation of Th1/Th2 cytokines in ovalbumin-sensitized mice. J Ethnopharmacol 137:1077–1082CrossRefGoogle Scholar
  21. Kuriyama I, Nakajima Y, Nishida H, Konishi T, Takeuchi T, Sugawara F, Yoshida H, Mizushina Y (2013) Inhibitory effects of low molecular weight polyphenolics from Inonotus obliquus on human DNA topoisomerase activity and cancer cell proliferation. Mol Med Rep 8:535–542Google Scholar
  22. Lee SH, Hwang HS, Yun JW (2009) Antitumor activity of water extract of a mushroom, Inonotus obliquus, against HT-29 human colon cancer cells. Phytother Res 23:1784–1789CrossRefGoogle Scholar
  23. Lee IK, Yun BS (2011) Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J Antibiot (Tokyo) 64:349–359CrossRefGoogle Scholar
  24. Lemieszek MK, Langner E, Kaczor J, Kandefer-Szerszen M, Sanecka B, Mazurkiewicz W, Rzeski W (2011) Anticancer effects of fraction isolated from fruiting bodies of Chaga medicinal mushroom, Inonotus obliquus(Pers.:Fr.) Pilát (Aphyllophoromycetideae): in vitro studies. Int J Med Mushrooms 13:131–143CrossRefGoogle Scholar
  25. Liang L, Zhang Z, Wang H (2009) Antioxidant activities of extracts and subfractions from Inonotus obliquus. Int J Food Sci Nutr 60:175–184CrossRefGoogle Scholar
  26. Lin SY, Yeh CC, Liang CH, Mau JL (2012) Preparation of Chaga medicinal mushroom, Inonotus obliquus-fermented rice using solid-state fermentation and its taste quality and antioxidant property. Int J Med Mushrooms 14:581–592CrossRefGoogle Scholar
  27. Lu X, Chen H, Dong P, Fu L, Zhang X (2010) Phytochemical characteristics and hypoglycaemic activity of fraction from mushroom Inonotus obliquus. J Sci Food Agric 90:276–280CrossRefGoogle Scholar
  28. Ma L, Chen H, Dong P, Lu X (2013) Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus. Food Chem 139:503–508CrossRefGoogle Scholar
  29. Mazurkiewicz W, Rydel K, Pogocki D, Lemieszek MK, Langner E, Rzeski W (2010) Separation of an aqueous extract Inonotus obliquus (Chaga). A novel look at the efficiency of its influence on proliferation of A549 human lung carcinoma cells. Acta Pol Pharm 67:397–406Google Scholar
  30. Mishra SK, Kang JH, Kim DK, Oh SH, Kim MK (2012) Orally administered aqueous extract of Inonotus obliquus ameliorates acute inflammation in dextran sulfate sodium (DSS)-induced colitis in mice. J Ethnopharmacol 143:524–532CrossRefGoogle Scholar
  31. Mu H, Zhang A, Zhang W, Cui G, Wang S, Duan J (2012) Antioxidative properties of crude polysaccharides from Inonotus obliquus. Int J Mol Sci 13:9194–9206CrossRefGoogle Scholar
  32. Nakajima Y, Nishida H, Nakamura Y, Konishi T (2009) Prevention of hydrogen peroxide-induced oxidative stress in PC12 cells by 3, 4-dihydroxybenzalacetone isolated from Chaga (Inonotus obliquus (persoon) Pilat). Free Radic Biol Med 47:1154–1161CrossRefGoogle Scholar
  33. Nomura M, Takahashi T, Uesugi A, Tanaka R, Kobayashi S (2008) Inotodiol, a lanostane triterpenoid, from Inonotus obliquus inhibits cell proliferation through caspase-3-dependent apoptosis. Anticancer Res 28:2691–2696Google Scholar
  34. Pan HH, Yu XT, Li T, Wu HL, Jiao CW, Cai MH, Li XM, Xie YZ, Wang Y, Peng T (2013) Aqueous extract from a Chaga medicinal mushroom, Inonotus obliquus (higher basidiomyetes), prevents herpes simplex virus entry through inhibition of viral-induced membrane fusion. Int J Med Mushrooms 15:29–38CrossRefGoogle Scholar
  35. Park YM, Won JH, Kim YH, Choi JW, Park HJ, Lee KT (2005) In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus. J Ethnopharmacol 101:120–128CrossRefGoogle Scholar
  36. Shibnev VA, Mishin DV, Garaev TM, Finogenova NP, Botikov AG, Deryabin PG (2011) Antiviral activity of Inonotus obliquus fungus extract towards infection caused by hepatitis C virus in cell cultures. Bull Exp Biol Med 151:612–614CrossRefGoogle Scholar
  37. Song FQ, Liu Y, Kong XS, Chang W, Song G (2013) Progress on understanding the anticancer mechanisms of medicinal mushroom: Inonotus obliquus. Asian Pac J Cancer Prev 14:1571–1578CrossRefGoogle Scholar
  38. Sun Y, Yin T, Chen XH, Zhang G, Curtis RB, Lu ZH, Jiang JH (2011) In vitro antitumor activity and structure characterization of ethanol extracts from wild and cultivated Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae). Int J Med Mushrooms 13:121–130CrossRefGoogle Scholar
  39. Sysoeva MA, Yumaeva LR, Kuznetsova OY, Ziyatdinova GK, Budnikov GK, Mel’nikova NB (2012) Study of the composition of biologically active compounds in chaga meal. Perspectives of application of chaga meal in pharmaceutical industry. Russ J Gen Chem 82:586–594CrossRefGoogle Scholar
  40. Van Q, Nayak BN, Reimer M, Jones PJ, Fulcher RG, Rempel CB (2009) Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay. J Ethnopharmacol 125:487–493CrossRefGoogle Scholar
  41. Won DP, Lee JS, Kwon DS, Lee KE, Shin WC, Hong EK (2011) Immunostimulating activity by polysaccharides isolated from fruiting body of Inonotus obliquus. Mol Cells 31:165–173Google Scholar
  42. Xiang Y, Xu X, Li J (2012) Chemical properties and antioxidant activity of exopolysaccharides fractions from mycelial culture of Inonotus obliquus in a ground corn stover medium. Food Chem 134:1899–1905CrossRefGoogle Scholar
  43. Xu HY, Sun JE, Lu ZM, Zhang XM, Dou WF, Xu ZH (2010) Beneficial effects of the ethanol extract from the dry matter of a culture broth of Inonotus obliquus in submerged culture on the antioxidant defence system and regeneration of pancreatic beta-cells in experimental diabetes in mice. Nat Prod Res 24: 42–553CrossRefGoogle Scholar
  44. Yan G, Jin G, Li L, Qin X, Zheng C, Li G (2011) Protective effects and mechanism of Inonotus obliquus on asthmatic mice. Zhongguo Zhong Yao Za Zhi 36:1067–1070Google Scholar
  45. Yoon TJ, Lee SJ, Kim EY, Cho EH, Kang TB, Yu KW, Suh HJ (2013) Inhibitory effect of chaga mushroom extract on compound 48/80-induced anaphylactic shock and IgE production in mice. Int Immunophramacol 15:666–670CrossRefGoogle Scholar
  46. Youn MJ, Kim JK, Park SY, Kim Y, Park C, Kim ES, Park KI, So HS, Park R (2009) Potential anticancer properties of the water extract of Inonotus [corrected] obliquus by induction of apoptosis in melanoma B16-F10 cells. J Ethnopharmacol 121:221–228CrossRefGoogle Scholar
  47. Yun JS, Pahk JW, Lee JW, Shin WC, Lee SY, Hong EK (2011) Inonotus obliquus protects against oxidative stress-induced apoptosis and premature senescence. Mol Cells 31:423–429CrossRefGoogle Scholar
  48. Zhang Y, Zhao Y, Cui H, Cao C, Guo J, Liu S (2011) Comparison of hypoglycemic activity of fermented mushroom of Inonotus obliquus rich in vanadium and wild-growing I. obliquus. Biol Trace Elem Res 144:1351–1357CrossRefGoogle Scholar
  49. Zhang N, Chen H, MA L, Zhang Y (2013) Physical modifications of polysaccharide from Inonotus obliquus and the antioxidant properties. Int J Biol Macromol 54:209–215CrossRefGoogle Scholar
  50. Zhao FQ, Yan L, Cui XH, Lin S, Wang C, Zhang H, Kang XY, Ji BS (2012) Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl4. Yao Xue Xue Bao 47:680–684Google Scholar
  51. Zheng W, Miao K, Liu Y, Zhao Y, Zhang M, Pan S, Dai Y (2010) Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and submerged culture strategies for up-regulating their production. Appl Microbiol Biotechnol 87:1237–1254CrossRefGoogle Scholar
  52. Zheng W, Zhao Y, Zheng X, Liu Y, Pan S, Dai Y, Liu F (2011a) Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus. Appl Microbiol Biotechnol 89:157–167CrossRefGoogle Scholar
  53. Zheng W, Zhang M, Zhao Y, Miao K, Pan S, Cao F, Dai Y (2011b) Analysis of antioxidant metabolites by solvent extraction from sclerotia of Inonotus obliquus (Chaga). Phytochem Anal 22:95–102CrossRefGoogle Scholar
  54. Zhong XH, Wang LB, Sun DZ (2011) Effects of inotodiol extracts from Inonotus obliquus on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line A549. Chin J Integr Med 17:218–223CrossRefGoogle Scholar
  55. Zhu L, Xu X (2013) Stimulatory effect of different lignocellulosic materials for phenolic compound production and antioxidant activity from Inonotus obliquus in submerged fermentation. Appl Biochem Biotechnol 169:2138–2152CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.San Diego State UniversitySan DiegoUSA

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