Abstract
Commercial enzymes can be obtained from natural sources or they can be produced recombinantly. A full enzyme characterization involved many methodologies but the spectrum of methods and the extent of characterization are related to the end use of the enzyme. Understanding the biochemical properties and mechanisms of enzymes are essential for research and development as well as for enzyme commercialization. These include among others the establishment of the optimum pH and stability of enzyme to different pH, the effects of temperature for the enzyme and the meaning of the apparent temperature optimum, the meaning of substrate specificity and enzyme specificity constant, the effects of small molecules including substrates, inhibitors and ions on enzyme activity, and last but not least the factors that are important for enzyme catalysis and enzyme mechanisms.
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An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-12397-4_13
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Belgasem, F., Salleh, H. (2015). Characterization of Recombinant Enzymes. In: Amid, A. (eds) Recombinant Enzymes - From Basic Science to Commercialization. Springer, Cham. https://doi.org/10.1007/978-3-319-12397-4_4
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DOI: https://doi.org/10.1007/978-3-319-12397-4_4
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