Abstract
We propose a simple method for forming fibroblast (NIH/3T3)-laden alginate lumen gels using a coaxial laminar flow for artificial vascularization, and was evaluated using confocal microscope As increasing the sodium alginate flow rates, the average outer diameters of the composed micro-tubes determined by the thickness of the injecting material, decreased. We mixed NIH/3T3 to gels and were alive during 4 days of cell culture, and this showed fibroblast cells to be well-organized and cultured maintaining the blood-vessel shape. The proposed technique could further be applied to an endothelial co-culturing system for mimicking the real blood vessel.
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© 2015 Springer International Publishing Switzerland
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Oh, D., Lee, S., Koo, KI., Seo, JM. (2015). Microscopic Tubular Cell Organization for Artificial Vascularization. In: Lacković, I., Vasic, D. (eds) 6th European Conference of the International Federation for Medical and Biological Engineering. IFMBE Proceedings, vol 45. Springer, Cham. https://doi.org/10.1007/978-3-319-11128-5_80
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DOI: https://doi.org/10.1007/978-3-319-11128-5_80
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-11127-8
Online ISBN: 978-3-319-11128-5
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