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Genetic Transformation Systems in Fungi, Volume 2 pp 131–138Cite as

Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus

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Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Conditional expression of a gene of interest is fundamental for elucidating any cellular function of its gene product. To achieve this in the human-pathogenic mould Aspergillus fumigatus, established doxycycline-dependent systems were demonstrated to be highly suitable, therefore supporting the aim to identify virulence determinants of this pathogenic saprobe.

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Authors and Affiliations

  1. Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Wasserturmstr. 3/5, 91054, Erlangen, Germany

    Michaela Dümig & Sven Krappmann Prof. Dr. rer. nat.

Authors
  1. Michaela Dümig
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  2. Sven Krappmann Prof. Dr. rer. nat.
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Corresponding author

Correspondence to Sven Krappmann Prof. Dr. rer. nat. .

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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Dümig, M., Krappmann, S. (2015). Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus . In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 2. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10503-1_10

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