Abstract
For many decades bovine serum has been used as an essential component for the growth of animal cells in culture. However, the combined disadvantages of variability in composition, cost but particularly the potential for contamination with viruses or prions has been the driver for the substitution of serum with a more defined and animal-component free media. For some cell lines substitution with just a few simple ingredients can provide an effective liquid media for growth. However, for a number of cell lines finding a suitable serum-free formulation for growth has been very challenging. Because of the complexity of these formulations statistically designed methods have been adopted to ensure a rational approach to media design. This, as well as the increasing availability of microbially-produced recombinant forms of animal proteins has been significant in the development of animal-component free and chemically defined media. Sometimes chemically-defined media have poorer characteristics for growth promotion than the serum-based formulations that they replace. However, incremental steps of improvement are possible by the addition of key ingredients or by adaptation of the cells to newly formatted serum-free media.
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References
Barnes D, Sato G (1980) Serum-free cell culture: a unifying approach. Cell 22(3):649–655
Castro PM, Hayter PM, Ison AP, Bull AT (1992) Application of a statistical design to the optimization of culture medium for recombinant interferon-gamma production by Chinese hamster ovary cells. Appl Microbiol Biotechnol 38:84–90
Chang TH, Steplewski Z, Koprowski H (1980) Production of monoclonal antibodies in serum free medium. J Immunol Methods 39:369–375
Dulbecco R, Freeman G (1959) Plaque production by the polyoma virus. Virology 8:396–397
Eagle H (1955) Nutrition needs of mammalian cells in tissue culture. Science 122:501–514
Eagle H (1959) Amino acid metabolism in mammalian cell cultures. Science 130:432–437
Florini JR, Roberts SB (1979) A serum-free medium for the growth of muscle cells in culture. In Vitro 15:983–992
Gopas J, Ono M, Princler G, Smith MR, Tainsky MA, Siddiqui MA, Wishniak O, Segal S, Kuwano M, Kung HF (1992) EGF receptor activity and mitogenic response of Balb/3 T3 cells expressing Ras and Myc oncogenes. EGF receptor activity in oncogene transformed cells. Cell Mol Biol 38:605–614
Gupta A, Harrison K, Maes D (2014) Understanding hydrolysate complexity. Genet Eng Biotechnol News 34:28–29
Ham RG (1965) Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci U S A 53:288–293
Ham RG, Mckeehan WL (1979) Media and growth requirements. Methods Enzymol 58:44–93
Hayashi I, Larner J, Sato G (1978) Hormonal growth control of cells in culture. In Vitro 14:23–30
Hodge G (2005) Media development for cell culture. Biopharm Int 18:54–57
Howard A, Udenigwe CC (2013) Mechanisms and prospects of food protein hydrolysates and peptide-induced hypolipidaemia. Food Funct 4:40–51
Hsueh HW, Moskowitz M (1973) A growth factor for animal cells derived from peptone. I. Isolation and characterization of the growth factor. Exp Cell Res 77:376–382
Jayme D, Watanabe T, Shimada T (1997) Basal medium development for serum-free culture: a historical perspective. Cytotechnology 23:95–101
Komolov IS, Fedotov VP (1978) Influence of insulin on mitotic rate in cultivated mammalian cells. Endocrinol Exp 12:43–48
Lee GM, Kim EJ, Kim NS, Yoon SK, Ahn YH, Song JY (1999) Development of a serum-free medium for the production of erythropoietin by suspension culture of recombinant Chinese hamster ovary cells using a statistical design. J Biotechnol 69:85–93
Luo Y, Pierce KM (2012) Development toward rapid and efficient screening for high performance hydrolysate lots in a recombinant monoclonal antibody manufacturing process. Biotechnol Prog 28:1061–1068
Mather JP, Sato GH (1979) The growth of mouse melanoma cells in hormone-supplemented, serum-free medium. Exp Cell Res 120:191–200
Merten OW (2002) Virus contaminations of cell cultures – a biotechnological view. Cytotechnology 39:91–116
Metcalfe HFR, Froud SJ (1994) The use of 2-hydroxy-2,4,6-cycloheptarin-1-one (tropolone) as a replacement for transferrin. In: Spier Re GJ, Berthold W (eds) Animal cell technology: products of today, prospects of tomorrow. Butterworth-Heinemann, Oxford, UK
Michiels J, Barbau J, Deboel S, Dessy S, Agathos SN, Schneider YJ (2011) Characterisation of beneficial and detrimental effects of a soy peptone, as an additive for CHO cell cultivation. Process Biochem 46:671–681
Morgan JF, Morton HJ, Parker RC (1950) Nutrition of animal cells in tissue culture; initial studies on a synthetic medium. Proc Soc Exp Biol Med 73:1–8
Morris AE, Schmid J (2000) Effects of insulin and LongR(3) on serum-free Chinese hamster ovary cell cultures expressing two recombinant proteins. Biotechnol Prog 16:693–697
Orly J, Sato G (1979) Fibronectin mediates cytokinesis and growth of rat follicular cells in serum-free medium. Cell 17:295–305
Pasupuleti VHC, Demain AL (2010) Applications of protein hydrolysates in biotechnology. In: Pasupuleti VADA (ed) Protein hydrolysates in biotechnology. Springer, Dordrecht
Schlaeger EJ (1996) The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties. J Immunol Methods 194:191–199
Siemensma A, Babcock J, Wilcox C, Huttinga H (2010) Towards an understanding of how protein hydrolysates stimulate more efficient biosynthesis in cultured cells. In: Pasupuleti VADA (ed) Protein hydrolysates in biotechnology. Springer, Dordrecht
Simmons JG, Hoyt EC, Westwick JK, Brenner DA, Pucilowska JB, Lund PK (1995) Insulin-like growth factor-I and epidermal growth factor interact to regulate growth and gene expression in IEC-6 intestinal epithelial cells. Mol Endocrinol 9:1157–1165
Simms E, Gazdar AF, Abrams PG, Minna JD (1980) Growth of human small cell (oat cell) carcinoma of the lung in serum-free growth factor-supplemented medium. Cancer Res 40:4356–4363
Stoker M, Macpherson I (1964) Syrian hamster fibroblast cell line Bhk21 and its derivatives. Nature 203:1355–1357
Vogt M, Dulbecco R (1960) Virus-cell interaction with a tumor-producing virus. Proc Natl Acad Sci U S A 46:365–370
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Butler, M. (2015). Serum and Protein Free Media. In: Al-Rubeai, M. (eds) Animal Cell Culture. Cell Engineering, vol 9. Springer, Cham. https://doi.org/10.1007/978-3-319-10320-4_8
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DOI: https://doi.org/10.1007/978-3-319-10320-4_8
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