Chapter

Genetic Transformation Systems in Fungi, Volume 1

Part of the series Fungal Biology pp 289-307

Date:

TALEN-Based Genome Editing in Yeast

  • Ting LiAffiliated withDepartment of Genetics, Development and Cell Biology, Iowa State University
  • , David A. WrightAffiliated withDepartment of Genetics, Development and Cell Biology, Iowa State University
  • , Martin H. SpaldingAffiliated withDepartment of Genetics, Development and Cell Biology, Iowa State University
  • , Bing YangAffiliated withDepartment of Genetics, Development and Cell Biology, Iowa State University Email author 

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Abstract

The development of nuclease-mediated Genome Editing represents a significant technological advance and may prove to be as important as PCR and DNA sequencing. Regardless, this technology puts the power of genome re-orchestration into the hands of research scientists for the first time and may lead to a paradigm shift in many biological fields. We present background information, so the reader has a greater understanding for the basis of this technology. We also discuss TAL effector nuclease (TALEN) as the latest engineered nuclease tool, its implementation and uses and present a yeast-based protocol that may be helpful in transitioning this technology to other fungal species.

Keywords

TAL effector nuclease (TALEN) Zinc finger nuclease Meganuclease Non-homologous end-joining (NHEJ) Homologous recombination (HR) Genetic engineering Gene editing Genome editing