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Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells

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Book cover Genetic Transformation Systems in Fungi, Volume 1

Part of the book series: Fungal Biology ((FUNGBIO))

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Abstract

Genetic transformation of specific cells is a key research tool in modern basic biological studies, as well as in biotechnology and gene therapy. Here we propose a principally new method enabling easy and effective delivery of plasmid DNA into the industrially important yeast species, Hansenula polymorpha, Pichia pastoris (this chapter), and Saccharomyces cerevisiae (data not presented). The transformation method is using a novel gene delivery system based on a comb-like oligoelectrolyte polymer consisting of the anionic backbone and dimethyl aminoethyl methacrylate (DMAEM)-based side branches.

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Acknowledgements

This work was partly supported by the grants from the WUBMRC (Ukraine-USA), CRDF (USA), and F-46 project of the National Academy of Sciences of Ukraine, as well as by the project funded by the Ministry of Education and Science of Ukraine.

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Authors and Affiliations

  1. Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences Ukraine, Drahomanov Street 14/16, Lviv, Ukraine, 79005

    Yevhen Filyak Ph.D., Nataliya Finiuk Ph.D. & Rostyslav Stoika Ph.D., Dr. Biol. Sci.

  2. Department of Organic Chemistry, Institute of Chemistry and Chemical Technologies, Lviv Polytechnic University, 12 Bandera Street, Lviv, Ukraine, 79103

    Nataliya Mitina Ph.D. & Alexander Zaichenko Ph.D.

Authors
  1. Yevhen Filyak Ph.D.
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  2. Nataliya Finiuk Ph.D.
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  3. Nataliya Mitina Ph.D.
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  4. Alexander Zaichenko Ph.D.
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  5. Rostyslav Stoika Ph.D., Dr. Biol. Sci.
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Corresponding author

Correspondence to Rostyslav Stoika Ph.D., Dr. Biol. Sci. .

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Editors and Affiliations

  1. Applied Biochemistry Department, DSM Biotechnology Center, Delft, The Netherlands

    Marco A. van den Berg

  2. Global Marker Technology Lab (DuPont-Pioneer), E.I.DuPont India Pvt Ltd, DuPont Knowledge Center, Hyderabad, Telangana, India

    Karunakaran Maruthachalam

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Filyak, Y., Finiuk, N., Mitina, N., Zaichenko, A., Stoika, R. (2015). Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells. In: van den Berg, M., Maruthachalam, K. (eds) Genetic Transformation Systems in Fungi, Volume 1. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-10142-2_20

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