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Sensors pp 13-17 | Cite as

Portable, Multispot, Label-Free Immunoassay on a Phantom Perfluorinated Plastic

  • Fabio Giavazzi
  • Matteo Salina
  • Erica Ceccarello
  • Mattia Bassi
  • Francesco Damin
  • Laura Sola
  • Marcella Chiari
  • Bice Chini
  • Roberto Cerbino
  • Tommaso Bellini
  • Marco BuscagliaEmail author
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 319)

Abstract

Despite the continuous advancements in bio-molecular detection methods and fluidic integration approaches, the realization of portable and high performance devices for diagnostic applications still presents major difficulties, mostly due to the need of combining adequate sensitivity with low cost of production, operational simplicity and rapidity. In this context, we have previously proposed a compact device composed of a smartphone and a custom-designed cradle, containing only a disposable sensing cartridge, a tiny magnetic stirrer and a few passive optical components. The detection principle is named Reflective Phantom Interface and is based on measuring the intensity of light reflected by the surface of an amorphous fluoropolymer substrate having a refractive index very similar to that of water. The reflectivity of dozens of spots is monitored in real time by the smartphone’s camera using the embedded flash LED as the illumination source. We tested the sensitivity and the repeatability of the combined device analyzing multiple spots of antibodies targeting an antigen commonly used as marker for diagnoses of HIV. Target concentrations as low as a few ng/ml can be rapidly and robustly determined by comparing the rate of increase of the signal after the addition of the sample with that measured after the subsequent addition of a standard solution with known concentration.

Keywords

Target Concentration Diagonal Surface Illumination Source Detection Principle Multiple Spot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Solvay Specialty Polymers R&D Center (Bollate, Italy) for providing the perfluorinated material and Dia.Pro Diagnostic Bioprobes (Milan, Italy) for providing the antibodies and antigens. This work was supported by the Cariplo Foundation (Grant 20069869), Italy and in part by EU (NAPES Project—NMP-2013-SMALL-7, project no. 604241).

References

  1. 1.
    F. Giavazzi, M. Salina, E. Ceccarello, A. Ilacqua, F. Damin, L. Sola, M. Chiari, B. Chini, R. Cerbino, T. Bellini, M. Buscaglia. A fast and simple label-free immunoassay based on a smartphone. Biosens. Bioelectron. 58, 395–402 (2014)CrossRefGoogle Scholar
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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Fabio Giavazzi
    • 1
  • Matteo Salina
    • 2
  • Erica Ceccarello
    • 1
  • Mattia Bassi
    • 3
  • Francesco Damin
    • 4
  • Laura Sola
    • 4
  • Marcella Chiari
    • 4
  • Bice Chini
    • 5
  • Roberto Cerbino
    • 1
  • Tommaso Bellini
    • 1
  • Marco Buscaglia
    • 1
    Email author
  1. 1.Dipartimento di Biotecnologie Mediche e Medicina TraslazionaleUniversità degli Studi di MilanoSegrateItaly
  2. 2.Proxentia S.r.l.SegrateItaly
  3. 3.Materials Science DepartmentSolvay Specialty Polymers Research and Development CenterBollateItaly
  4. 4.Istituto di Chimica del Riconoscimento Molecolare—CNRMilanoItaly
  5. 5.Istituto di Neuroscienze—CNRMilanoItaly

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