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Progress Toward the Development of a Lytic Bacteriophages-Based Impedance Microbiology for Agro-Food Application

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Sensors

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 319))

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Abstract

A novel approach to impedance microbiology (IM) has been under investigation. In our approach, solution resistance variations will be generated from bacteriophage (phage) lyses of host cell and the consequent release of endoplasmic material. To sensitively detect the lysis, bacteria cells have to be concentrated in a micro-electrochemical cell so that dilution of the released conductive molecules will be minimised. Therefore, the detection principle has been developed in conjunction with a sample preparation method for bacteria capture and concentration based on phage functionalised paramagnetic nanobeads and magnetic concentration. Escherichia coli has been used as target bacteria and the beads have been functionalised with selective lytic phages. The method has a potential detection limit slightly below 10 CFU/chamber. The whole assay detection time is bound to be below 30 min as it is strictly linked to the phages lytic cycle. The assay has potential for integration in automated systems.

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References

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Acknowledgments

The RESTATE project (Marie Curie Cofund, Grant agreement no. 267224) is acknowledged for the financial support. The authors thank E. Fazio (Messina University) for the support with the Raman Spectroscopy measurement.

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Authors and Affiliations

  1. Center for Materials and Microsystems, Fondazione Bruno Kessler, Trento, Italy

    Alessia Mortari & Leandro Lorenzelli

  2. Department of Environmental and Biological Sciences, University of Messina, Messina, Italy

    Marco Nicolò, Salvatore Guglielmino & Laura Maria De Plano

Authors
  1. Alessia Mortari
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  2. Leandro Lorenzelli
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  3. Marco Nicolò
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  4. Salvatore Guglielmino
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  5. Laura Maria De Plano
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Corresponding author

Correspondence to Alessia Mortari .

Editor information

Editors and Affiliations

  1. Faculty of Bioscience and Technologies for Food Agriculture and Environment, University of Teramo, Mosciano S.A., Teramo, Italy

    Dario Compagnone

  2. Institute of Applied Physics IFAC-CNR, Firenze, Italy

    Francesco Baldini

  3. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. University of Cassino, Cassino, Italy

    Giovanni Betta

  5. CNR, Head of Inst. of Microelectronics a, Lecce, Italy

    Pietro Siciliano

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© 2015 Springer International Publishing Switzerland

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Mortari, A., Lorenzelli, L., Nicolò, M., Guglielmino, S., De Plano, L. (2015). Progress Toward the Development of a Lytic Bacteriophages-Based Impedance Microbiology for Agro-Food Application. In: Compagnone, D., Baldini, F., Di Natale, C., Betta, G., Siciliano, P. (eds) Sensors. Lecture Notes in Electrical Engineering, vol 319. Springer, Cham. https://doi.org/10.1007/978-3-319-09617-9_15

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  • DOI: https://doi.org/10.1007/978-3-319-09617-9_15

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09616-2

  • Online ISBN: 978-3-319-09617-9

  • eBook Packages: EngineeringEngineering (R0)

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