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Imaging of Complications in Atherosclerosis: Thrombosis and Platelet Aggregation

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Cardiovascular Imaging

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Abstract

The thrombotic cellular mechanisms associated with cardiovascular events remain unclear, largely because of an inability to visualize thrombus formation in vivo. In this chapter, bio-imaging approach is introduced, mainly using in vivo imaging technique based on single-photon and multiphoton microscopy. Recent progress in optics enabled us to identify single-platelet behavior in the developing thrombus in living animals. In the physiological and pathological conditions, complex cellular interplay takes place, and bio-imaging can directly elucidate cross talks among multiple cell types. We visualized that rapidly developing thrombi composed of discoid platelets were triggered by reactive oxygen species photochemically induced by laser irradiation, and developed thrombi occluded blood vessels. Inflammatory cytokine and adhesion molecules dynamically control these processes. Intravital imaging can be a powerful tool to analyze thrombus formation and evaluate the therapeutic strategies against thrombotic diseases.

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Author information

Authors and Affiliations

  1. Department of Cardiovascular Medicine Translational Systems Biology and Medicine Initiative, The University of Tokyo, Tokyo, Japan

    Satoshi Nishimura MD, PhD

  2. Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan

    Satoshi Nishimura MD, PhD

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  1. Satoshi Nishimura MD, PhD
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Correspondence to Satoshi Nishimura MD, PhD .

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Editors and Affiliations

  1. Cardiovascular Medicine, Brigham and Women’s Hospital Harvard Medical School, Boston, Massachusetts, USA

    Elena Aikawa

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Nishimura, S. (2015). Imaging of Complications in Atherosclerosis: Thrombosis and Platelet Aggregation. In: Aikawa, E. (eds) Cardiovascular Imaging. Springer, Cham. https://doi.org/10.1007/978-3-319-09268-3_8

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  • DOI: https://doi.org/10.1007/978-3-319-09268-3_8

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09267-6

  • Online ISBN: 978-3-319-09268-3

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