Abstract
With the development and application of new technologies, biotechnology of forage and turf species has been rapid development in recent years in China, mainly in the field of omics and bioinformatics, new gene discovery and genetic engineering, and molecular marker technology. Omics and bioinformatics are essential to understand the molecular systems that underlie various plant functions. The rapid development of next-generation sequencing technology (NGS) makes genomics research undergoes a tremendous change, and promotes the development and application of bioinformatics tools. In recent years, along with the uses of genomics and bioinformatics tools, the studies of forage and turf species have developed rapidly, which reflected mainly in genomics and transcriptomics. The development of omics resources has progressed to address particular biological properties of individual forage and turf species, and also provide resources to explore new genes including new function or new sequence. The application of reverse genetics in some forage species by construction of mutant library also accelerated the new gene discovery. Genetic engineering in forage and turf species mainly focused on plant genetic improvement of biotic and abiotic stresses, quality, and transgenic vaccine. Environmental safety and broad-spectrum resistance were worth considering when the genetic engineering vector was constructed. Research in the field of molecular marker technology has been developed from the first- and second-generation to the third-generation. Molecular markers have been applied to analyze genetic diversity, marker-assisted breeding, genetic linkage map construction, QTL mapping, and germplasm or variety fingerprints. From these perspectives, we provide this review of the emerging aspects of forage and turf species researches in biotechnology based on the recent technological advances.
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References
Bao AK, Wang YW, Xi JJ, Liu C, Zhang JL, Wang SM (2014) Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1–1 enhances salt and drought tolerance in transgenic Lotus corniculatus L. by increasing cations accumulation. Funct Plant Biol 41:203–214
Chen SY, Huang X, Yan XQ, Liang Y, Wang YZ, Li XF, Peng XJ, Ma XY, Zhang LX, Cai YY, Ma T, Cheng LQ, Qi DM, Zheng HJ, Yang XH, Li XX, Liu GS (2013) Transcriptome analysis in sheepgrass (Leymus chinensis): a dominant perennial grass of the Eurasian Steppe. PLoS One 8(7):e67974
Chen SY, Cai YY, Zhang LX, Yan XQ, Cheng LQ, Qi DM, Zhou QY, Li XX, Liu GS (2014) Transcriptome analysis reveals common and distinct mechanisms for sheepgrass (Leymus chinensis) responses to defoliation compared to mechanical wounding. PLoS One 9(2):e89495
Cheng LQ, Li XX, Huang X, Ma T, Liang Y, Ma XY, Peng XJ, Jia JT, Chen SY, Chen Y, Deng B, Liu GS (2013) Overexpression of sheepgrass R1-MYB transcription factor LcMYB1 confers salt tolerance in transgenic Arabidopsis. Plant Physiol Bioch 70:252–260
Dong J, Wang XM, Wang Z, Gao H, Sun GZ (2012) Cloning and analysis of dihydroflavonol reductase (DFR) gene from Medicago sativa. Acta Pratac Sin 21(2):123–132
Guo Q, Wang P, Ma Q, Zhang JL, Bao AK, Wang SM (2012a) Selective transport capacity for K + over Na + is linked to the expression levels of PtSOS1 in halophyte Puccinellia tenuiflora. Funct Plant Biol 39:1047–1057
Guo QQ, Zhang ZL, Jiang SJ, Ma JT, Xue WT, Wu YM (2012b) Expression of an Avian Influenza Virus (H5N1) Hemagglutinin gene in transgenic Lotus corniculatus. Plant Mol Biol Rep 30:1117–1124
Hu K, Yan XF, Li D, Tang XM, Yang H, Wang Y et al (2013) Genetic improvement of perennial ryegrass with low lignin content by silencing genes of CCR and CAD. Acta Pratac Sin 22(5):72–83
Huang LK, Zhang XQ, Xie WG, Zhang J, Cheng L, Yan HD (2012) Molecular diversity and population structure of the forage grass Hemarthria compressa (Poaceae) in south China based on SRAP markers. Genet Mol Res 11(3):2441–2450
Huo S, Chen H, Zhu QH, Xie XM (2012) Cloning of a 4CL gene fragment and construction of a RNAi expression vector of Pennisetum purpureum. Acta Pratac Sin 21(1):296–301
Jia HL, Wang XM, Gao HW, Dong J, Wang YQ, Liu JN et al (2012) Cloning the gene of γ-tocopherol methyltransferase from alfalfa and expression analysis in adverse situations. Acta Pratac Sin 21(6):198–206
Jiang J, Yang BL, Xia T, Yu SM, Wu YN (2011) Analysis of genetic diversity of salt tolerant alfalfa germplasms. Acta Pratac Sin 20(5):119–125
Jiang LF, Zhang XQ, Ma X, Huang LK, Xie WG, Ma YM, Zhao YF (2013) Identification of orchardgrass (Dactylis glomerata L.) cultivars by using simple sequence repeat markers. Genet Mol Res 12(4):5111–5123
Li D, Su Z, Dong J, Wang T (2009) An expression database for roots of the model legume Medicago truncatula under salt stress. BMC Genomics 10:517
Li D, Zhang Y, Hu X, Shen X, Ma L, Su Z, Wang T, Dong J (2011) Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses. BMC Plant Biol 11:109
Li XX, Gao Q, Liang Y, Ma T, Cheng LQ, Qi DM, Liu H, Xu X, Chen SY, Liu GS (2013a) A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis. Plant Physiol Bioch 64:52–59
Li XX, Hou SL, Gao Q, Zhao PC, Chen SY, Qi DM, Lee BH, Cheng LQ, Liu GS (2013b) LcSAIN1, a novel salt-induced gene from sheepgrass, confers salt stress tolerance in transgenic Arabidopsis and rice. Plant Cell Physiol 54(7):1172–1185
Liu RX, Yu LQ, Zhang Y, Wu D, Yun JF (2010) Clustering and evaluation of alfalfa germplasms with different fall dormancy levels by RAPD markers. Acta Agrestia sinica 18(1):108–114
Liu J, Zhao Q, Yang ZM (2012) Identification of nine Cynodon dactylon varieties by SRAP molecular markers. Chin J grassland 4:21–25
Liu Z, Ma L, Nan Z, Wang Y (2013a) Comparative transcriptional profiling provides insights into the evolution and development of the zygomorphic flower of Vicia sativa (Papilionoideae). PLoS One 8(2):e57338
Liu Z, Chen T, Ma L, Zhao Z, Zhao PX, Nan Z, Wang Y (2013b) Global transcriptome sequencing using the Illumina platform and the development of EST-SSR markers in autotetraploid alfalfa. PLoS One 8(12):e83549
Ma X, Gu XY, Chen TT, Chen SY, Huang LK, Zhang XQ (2013a) Genetic relationships between Lolium (Poaceae) species revealed by RAPD markers. Genet Mol Res 12(3):3246–3255
Ma Q, Li XY, Yuan HJ, Hu J, Wei L, Bao AK, Zhang JL, Wang SM (2013b) ZxSOS1 is essential for long-distance transport and spatial distribution of Na + and K + in the xerophyte Zygophyllum xanthoxylum. Plant Soil 374:661–676
Pang Y, Peel GJ, Sharma SB, Tang Y, Dixon RA (2008) A transcript profiling approach reveals an epicatechin-specific glucosyltransferase expressed in the seed coat of Medicago truncatula. PNAS 105(37):14210–14215
Pang Y, Wenger JP, Saathoff K, Peel GJ, Wen J, Huhman D, Allen SN, Tang Y, Cheng X, Tadege M, Ratet P, Mysore KS, Sumner LW, Marks MD, Dixon RA (2009) A WD40 repeat protein from Medicago truncatula is necessary for tissue-specific anthocyanin and proanthocyanidin biosynthesis but not for trichome development. Plant Physiol 151(3):1114–1129
Pang Y, Cheng X, Huhman D, Ma J, Peel JG, Yonekura-Sakakibara K et al (2013) Medicago glucosyltransferase UGT72L1: potential roles in proanthocyanidin biosynthesis. Planta 238(1):139–154
Peng XJ, Ma XY, Fan WH, Su M, Cheng LQ, Alam I, Lee BH, Qi DM, Shen SH, Liu GS (2011) Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis. Plant Cell Rep 30:1493–1502
Peng XJ, Zhang LX, Zhang LX, Liu ZJ, Cheng LQ, Yang Y, Shen SH, Chen SY, Liu GS (2013) The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis. Plant Cell Tiss Org 113:245–256
Rao JP, Agrawal P, Mohammad R, Rao SK, Reddy GR, Dechamma HJ, S Suryanarayana VV (2012) Expression of VP1 protein of serotype A and O of foot-and-mouth disease virus in transgenic sunnhemp plants and its immunogenicity for guinea pigs. Acta Virol 56(2):91–99
Shen X, Martens, Chen M, Li D, Dong J, Wang T (2010) Cloning and characterization of a functional flavanone-3-hydroxylase gene from Medicago truncatula. Mol Biol Rep 37(7):3283–3289
Su M, Li XX, Li XF, Cheng LQ, Qi DM, Chen SY, Liu GS (2013) Molecular characterization and expression analysis of a sheepgrass sucrose transporter LcSUT1 after defoliation. Plant Mol Biol Rep 31(5):1184–1191
Sun Y, Long R, Kang J, Zhang T, Zhang Z, Zhou H, Yang Q (2013) Molecular cloning and characterization of three isoprenyl diphosphate synthase genes from alfalfa. Mol Biol Rep 40(2):2035–2044
Tang R, Zhang XQ, Li YH, Xie XM (2014) Cloning and in silico analysis of a cinnamyl alcohol dehydrogenase gene in Pennisetum purpureum. J Genet 93(1):145–158
Tong ZY, Xie C, Ma L, Liu LP, Jin YS, Dong JL, Wang T (2014) Co-expression of bacterial aspartate kinase and adenylylsulfate reductase genes substantially increases sulfur amino acid levels in transgenic alfalfa (Medicago sativa L.). PLoS One 9(2):e88310
Wang DM, Zhu JB, Peng M, Zhou P (2008) Induction of a protective antibody response to FMDV in mice following oral immunization with transgenic Stylosanthes spp. as a feedstuff additive. Transgenic Res 17(6):1163–1170
Wu GQ, Xi JJ, Wang Q, Bao AK, Ma Q, Zhang JL, Wang SM (2011) The ZxNHX gene encoding tonoplast Na+/H+ antiporter from the xerophyte Zygophyllum xanthoxylum plays important roles in response to salt and drought. J Plant Physiol 168:758–767
Xie WG, Zhang XQ, Cai HW, Liu W, Peng Y (2010) Genetic diversity analysis and transferability of cereal EST-SSR markers to orchardgrass (Dactylis glomerata L.). Biochem Syst Ecol 38(4):740–749
Xie WG, Zhang XQ, Cai HW, Huang LK, Peng Y, Ma X (2011) Genetic maps of SSR and SRAP markers in diploid orchardgrass (Dactylis glomerata L.) using the pseudo-testcross strategy. Genome 54(3):212–221
Xie C, Zhang R, Qu Y, Miao Z, Zhang Y, Shen X, Wang T, Dong J (2012) Overexpression of MtCAS31 enhances drought tolerance in transgenic Arabidopsis by reducing stomatal density. New Phytol 195(1):124–135
Zhang XY, He JG, Sun XH, Wu JX (2011) Transformation of Lolium perenne with a fructan: fructan 1-fructosyltransferase gene from Agropyron cristatum and enhancement of drought tolerance in transgenic plants. Acta Pratac Sin 20(1):111–118
Acknowledgements
This work was supported by National Basic Research Program of China (“973”, 2014CB138704), the National High Technology Research and Development Program of China (“863”, 2011AA100209).
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Chen, S. et al. (2015). Advances in Forage and Turf Biotechnology in China. In: Budak, H., Spangenberg, G. (eds) Molecular Breeding of Forage and Turf. Springer, Cham. https://doi.org/10.1007/978-3-319-08714-6_10
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DOI: https://doi.org/10.1007/978-3-319-08714-6_10
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