Novel Approach for Investigation of Neuronal Alterations Following Ischemic Stroke in a Rat Model

Abstract

Ischemic stroke is a complex pathological trauma involving a multitude of detrimental processes. Due to the complexity of both the neural networks in the brain and the pathological process following stroke, novel methods to obtain knowledge about ischemic stroke are needed. One recently suggested model is to utilize intracortical (IC) microwire electrode-arrays to record from ischemic cortical tissue. In the present work we report on refinements made to the model, including design of a novel IC electrode-array and methods for quantification and analysis of the obtained IC recordings. IC recordings were obtained from one Sprague-Dawley rat prior to induction of ischemic stroke and in a follow-up period of 150 min post-stroke. The results showed that the refinements of the model resulted in a more intuitive way of interpreting the progression of ischemic stroke. Future enhancements of the model should concern features that focus on the spatial dynamics within the ischemia affected neural networks. In addition to assisting in generating knowledge about the pathology of ischemic stroke the model may be useful for optimization of rehabilitative electrical stimulation protocols.