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Transcriptome Analysis of Musa and its Applications in Banana Improvement

  • S Backiyarani
  • S UmaEmail author
  • M. S. Saraswathi
  • A Chandrasekar
Chapter
Part of the Sustainable Development and Biodiversity book series (SDEB, volume 2)

Abstract

Banana, with its unique characteristics like varied ploidy levels, parthenocarpy, sterility, vegetative propagation and biparental cytoplasmic inheritance, serves as a powerful model for understanding the genetics of these traits where the use of other two model species namely Arabidopsis and rice is not possible/appropriate. Transcriptome analysis is proven to be an efficient tool for understanding the molecular mechanisms involved under specific physiological conditions. In banana, different approaches like SSH, cDNA-AFLP, ESTs, and microarray have been tried to understand the molecular mechanism involved during fruit ripening and various biotic and abiotic stress conditions. The detailed outcome of the results, merits and demerits of these approaches are discussed. Recent technologies of next generation sequencing (NGS) have also been used to profile the transcriptome under various biotic and abiotic stress conditions. The generation of enormous amount of transcriptome sequence data resulted in development of genetic markers like EST-SSRs and SNPs etc. The genes identified through these approaches are also being used in developing resistant/tolerant banana varieties through transgenic approaches. The importance of using double reference sequences (A and B genome) for transcriptome mapping is emphasized. The potential application of transcriptome analyses in banana improvement programme are discussed in this chapter.

Keywords

Musa spp. NGS transcriptomics Host pathogen interaction 

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • S Backiyarani
    • 1
  • S Uma
    • 1
    Email author
  • M. S. Saraswathi
    • 1
  • A Chandrasekar
    • 1
  1. 1.Crop improvementNational Research Centre for Banana, (ICAR)TiruchirapalliIndia

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