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Production of Nitric Oxide by Marine Unicellular Red Tide Phytoplankton, Chattonella marina

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Nitric Oxide in Plants: Metabolism and Role in Stress Physiology

Abstract

In this chapter, we show several lines of evidence for the production of nitric oxide (NO) by unicellular red tide phytoplankton Chattonella marina under the normal growth conditions. Chemiluminescence (CL) assay suggested that C. marina produced NO in a cell-number-dependent manner, and the level of NO decreased by the addition of carboxy-PTIO, a specific NO scavenger. NO generation by C. marina was also confirmed by a spectrophotometric assay based on the measurement of the diazo-reaction positive substances (NOx) and by fluorometric assay using highly specific fluorescent indicator of NO. Furthermore, the NO level in C. marina was significantly decreased by l-NAME, a specific NO synthase (NOS) inhibitor. The addition of l-arginine increased the NO level, whereas NaNO2 had no effect. These results suggest that a NOS-like enzyme is mainly responsible for NO generation in C. marina.

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Authors and Affiliations

  1. Jeju Center, Korea Basic Science Institute (KBSI), Smart Building 1F, 2170-2 Jeju Science Park, Ara-dong, Jeju-Si, Jeju Special Self-Governing Province, 690-756, Korea

    Daekyung Kim

  2. Faculty of Fisheries, Division of Biochemistry, Nagasaki University, Bunkyo-machi 1-14, Nagasaki, 852-8521, Japan

    Tatsuya Oda

Authors
  1. Daekyung Kim
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  2. Tatsuya Oda
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Correspondence to Tatsuya Oda .

Editor information

Editors and Affiliations

  1. Faculty of Science, Department of Biology, University of Tabuk, Tabuk, Saudi Arabia

    M. Nasir Khan

  2. Faculty of Science, Department of Biology, University of Tabuk, Tabuk, Saudi Arabia

    Mohammad Mobin

  3. Plant Physiology Section,Department of Botany, Aligarh Muslim University, Aligarh, India

    Firoz Mohammad

  4. Departamento de Bioquímica Biol. Cellular y Molecular de Plantas, Estación Experimental del Zaidín Consejo Superior de Investigaciones Científicas, Granada, Spain

    Francisco J. Corpas

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Kim, D., Oda, T. (2014). Production of Nitric Oxide by Marine Unicellular Red Tide Phytoplankton, Chattonella marina . In: Khan, M., Mobin, M., Mohammad, F., Corpas, F. (eds) Nitric Oxide in Plants: Metabolism and Role in Stress Physiology. Springer, Cham. https://doi.org/10.1007/978-3-319-06710-0_5

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