Platinum Nanoparticles with Adsorptive Layer of Chlorella vulgaris Polysaccharides Inactivate Tumor Cells of Ascitic Ehrlich Carcinoma, Ovarian Cancer and Leukemia
In comparison (with) nanogold, not much work on the application of nanoplatinum in cancer therapy is known. The purpose of this work is to investigate the aggregation of platinum nanoparticles with different cancer cells and its effects on cells’ vital activity.
The “green” synthesis of platinum nanoparticles from hexachloroplatinic acid solution was conducted, using as a reducing agent ascorbic acid for platinum nanoparticles, and as a reducing stabilizer—polysaccharides of Chlorella for the same nanoparticles modified by the polysaccharide adsorption layer. Here, we take into consideration the known properties of Chlorella’s polysaccharides as immunostimulators, inhibitors of proliferation, and activators of apoptosis of cancer cells.
It was studied the interaction of NP PtPS with cancer cells (ascitic Ehrlich carcinoma, ovarian cancer A2780 human line, leukemia - transformed β-lymphocytes-cells Namalwa line, and for comparison - with lymphocytes of healthy human) on the basis of a complex of methods, including studies of heterocoagulation of cells and particles and kinetics of cell death (cytotoxic effect).
This study showed that for the first 20 minutes of contact were died over 50% cells of ovarian cancer and Ehrlich carcinoma. For leukemic cells, such time is 2 h. The selectivity of NP PtPS action was established—more than 90 % of healthy lymphocytes maintain their viability after 2 h of contact. Bioconjugates of platinum nanoparticles with polysaccharides can be the basis of the perspective drug for cancer therapy.
KeywordsOvarian Cancer Adsorption Layer Platinum Nanoparticles Human Ovarian Cancer Cell Ascitic Ehrlich Carcinoma
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