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Affinity-Mass Spectrometry Approaches for Elucidating Structures and Interactions of Protein–Ligand Complexes

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

Affinity-based approaches in combination with mass spectrometry for molecular structure identification in biological complexes such as protein–protein, and protein–carbohydrate complexes have become popular in recent years. Affinity-mass spectrometry involves immobilization of a biomolecule on a chemically activated support, affinity binding of ligand(s), dissociation of the complex, and mass spectrometric analysis of the bound fraction. In this chapter the affinity-mass spectrometric methodologies will be presented for (1) identification of the epitope structures in the Abeta amyloid peptide, (2) identification of oxidative modifications in proteins such as nitration of tyrosine, (3) determination of carbohydrate recognition domains, and as (4) development of a biosensor chip-based mass spectrometric system for concomitant quantification and identification of protein–ligand complexes.

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Acknowledgments

The author would like to acknowledge Professor Michael Przybylski, Dr. Mihaela Stumbaum, Dr. Roxana Iacob, Dr. Irina Perdivara, Dr. Marilena Manea, and Adrian Moise for interesting topic, useful discussions, and expertise. Our research was supported in part by the Deutsche Forschungsgemeinschaft, Bonn, Germany (PR-175-14-1 and FG-753), EC for the GlycoHIT consortium, German Academic Exchange Service (DAAD), and the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, project number PN-II-RU-TE-2011-3-0038.

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Authors and Affiliations

  1. Laboratory of Biochemistry, Department of Chemistry, Al. I. Cuza University of Iasi, Carol I Boulevard, No. 11, 700506, Iasi, Romania

    Brînduşa Alina Petre

  2. Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, 78457, Constance, Germany

    Brînduşa Alina Petre

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  1. Brînduşa Alina Petre
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Correspondence to Brînduşa Alina Petre .

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  1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Alisa G. Woods

  2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Costel C. Darie

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Petre, B.A. (2014). Affinity-Mass Spectrometry Approaches for Elucidating Structures and Interactions of Protein–Ligand Complexes. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_7

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